https://scholars.lib.ntu.edu.tw/handle/123456789/626424
標題: | Rat Pheochromocytoma PC12 Cells in Culture | 作者: | Chen, Pin Chun CHIH-TIEN WANG |
關鍵字: | Dense-core vesicle | Fusion pore kinetics | Norepinephrine | PC12 cell culture | Proximity ligation assay with immunostaining | Rat pheochromocytoma | Regulated exocytosis | Single-cell biochemistry assay | Single-vesicle amperometry | 公開日期: | 1-一月-2023 | 出版社: | Humana Press Inc. | 卷: | 2565 | 起(迄)頁: | 3-15 | 來源出版物: | Methods in Molecular Biology | 摘要: | PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626424 | ISBN: | 978-1-0716-2670-2 978-1-0716-2671-9 |
ISSN: | 10643745 | DOI: | 10.1007/978-1-0716-2671-9_1 |
顯示於: | 分子與細胞生物學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。