https://scholars.lib.ntu.edu.tw/handle/123456789/626599
標題: | Mass spectrometry-based metabolomic signatures of coral bleaching under thermal stress | 作者: | Pei, Ji-Ying Yu, Wen-Feng Zhang, Jing-Jing Kuo, Ting-Hao Chung, Hsin-Hsiang Hu, Jun-Jie CHENG-CHIH HSU Yu, Ke-Fu |
關鍵字: | Bleaching; Lipid metabolism; Mass spectrometry; Metabolomics; Thermal stress | 公開日期: | 十一月-2022 | 出版社: | SPRINGER HEIDELBERG | 卷: | 414 | 期: | 26 | 起(迄)頁: | 7635 | 來源出版物: | Analytical and bioanalytical chemistry | 摘要: | Coral bleaching caused by climate change has resulted in large-scale coral reef decline worldwide. However, the knowledge of physiological response mechanisms of scleractinian corals under high-temperature stress is still challenging. Here, untargeted mass spectrometry-based metabolomics combining with Global Natural Product Social Molecular Networking (GNPS) was utilized to investigate the physiological response of the coral species Pavona decussata under thermal stress. A wide variety of metabolites (including lipids, fatty acids, amino acids, peptides, osmolytes) were identified as the potential biomarkers and subjected to metabolic pathway enrichment analysis. We discovered that, in the thermal-stressed P. decussata coral holobiont, (1) numerous metabolites in classes of lipids and amino acids significantly decreased, indicating an enhanced lipid hydrolysis and aminolysis that contributed to up-regulation in gluconeogenesis to meet energy demand for basic survival; (2) pantothenate and panthenol, two essential intermediates in tricarboxylic acid (TCA) cycle, were up-regulated, implying enhanced efficiency in energy production; (3) small peptides (e.g., Glu-Leu and Glu-Glu-Glu-Glu) and lyso-platelet-activating factor (lysoPAF) possibly implicated a strengthened coral immune response; (4) the down-regulation of betaine and trimethylamine N-oxide (TMAO), known as osmolyte compounds for maintaining holobiont homeostasis, might be the result of disruption of coral holobiont. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626599 | ISSN: | 1618-2642 | DOI: | 10.1007/s00216-022-04294-y |
顯示於: | 化學系 |
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