https://scholars.lib.ntu.edu.tw/handle/123456789/626680
標題: | Multiomic characterization and drug testing establish circulating tumor cells as an ex vivo tool for personalized medicine | 作者: | Chen, Jia-Yang Chou, Hsu-Huan Lim, Syer Choon Huang, Yen-Jang Lai, Kuan-Chen Guo, Chin-Lin Tung, Chien-Yi Su, Chung-Tsai Wang, Jocelyn Liu, Edward Han, Hsiao-Fen Yeh, Po-Ying Hu, Chun-Mei Dunn, Alexander R Frank, Curtis W YI-CHUN WU Yang, Muh-Hwa Chang, Ying-Chih |
關鍵字: | Cancer; Omics; Precision medicine; Transcriptomics | 公開日期: | 21-十月-2022 | 出版社: | CELL PRESS | 卷: | 25 | 期: | 10 | 起(迄)頁: | 105081 | 來源出版物: | iScience | 摘要: | Matching the treatment to an individual patient's tumor state can increase therapeutic efficacy and reduce tumor recurrence. Circulating tumor cells (CTCs) derived from solid tumors are promising subjects for theragnostic analysis. To analyze how CTCs represent tumor states, we established cell lines from CTCs, primary and metastatic tumors from a mouse model and provided phenotypic and multiomic analyses of these cells. CTCs and metastatic cells, but not primary tumor cells, shared stochastic mutations and similar hypomethylation levels at transcription start sites. CTCs and metastatic tumor cells shared a hybrid epithelial/mesenchymal transcriptome state with reduced adhesive and enhanced mobilization characteristics. We tested anti-cancer drugs on tumor cells from a metastatic breast cancer patient. CTC responses mirrored the impact of drugs on metastatic rather than primary tumors. Our multiomic and clinical anti-cancer drug response results reveal that CTCs resemble metastatic tumors and establish CTCs as an ex vivo tool for personalized medicine. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626680 | ISSN: | 25890042 | DOI: | 10.1016/j.isci.2022.105081 |
顯示於: | 分子與細胞生物學研究所 |
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