https://scholars.lib.ntu.edu.tw/handle/123456789/626791
標題: | Bromelain activates the AMP-activated protein kinase-autophagy pathway to alleviate hepatic lipid accumulation | 作者: | Hu, PA Hsu, MC Chen, SH CHERYL CHIA-HUI CHEN Kou, YR JENQ-WEN HUANG TZONG-SHYUAN LEE |
關鍵字: | AMPK; Autophagy; Bromelain; NAFLD; NONALCOHOLIC FATTY LIVER; METABOLIC SYNDROME; DISEASE; THERAPIES | 公開日期: | 2022 | 出版社: | DIGITAL COMMONS BEPRESS | 卷: | 30 | 期: | 3 | 起(迄)頁: | 357 | 來源出版物: | JOURNAL OF FOOD AND DRUG ANALYSIS | 摘要: | Bromelain, a cysteine protease found in pineapple, is known to exert protective effects against non-alcoholic fatty liver disease (NAFLD); however, the underlying mechanism is unclear. In this study, we aimed to investigate the molecular mechanisms underlying the beneficial effects of bromelain using in vivo and in vitro models. C57BL/6 mice were fed a high-fat diet (HFD) with or without bromelain (20 mg/kg/day) for 12 weeks. We found that treatment with bromelain alleviated hepatic lipid accumulation accompanied by the activation of AMP-activated protein kinase (AMPK) and autophagy flux, as evidenced by the elevated levels of phosphorylated AMPK, ATG5, ATG7, LC3-II, and lysosomeassociated membrane protein 2 (LAMP2), and the decreased levels of p62 in the liver of HFD-fed mice. In human hepatoma Huh 7 cells, bromelain prevented oleic acid (OA)-induced lipid accumulation and increased the levels of phosphorylated AMPK, ATG5, ATG7, LC3-II, and LAMP2 but decreased the levels of p62. Inhibition of AMPK and autophagy flux by specific inhibitors or small interfering RNAs suppressed bromelain-mediated protective effect on lipid accumulation. Moreover, inhibition of AMPK activity abolished the activation of autophagy flux in OA-treated hepatocytes. Collectively, these findings suggest a new molecular mechanism involving the AMPK-autophagy pathway through which bromelain confers protection against the deregulation of lipid metabolism in the liver. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626791 | ISSN: | 1021-9498 | DOI: | 10.38212/2224-6614.3416 |
顯示於: | 醫學系 |
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