https://scholars.lib.ntu.edu.tw/handle/123456789/626973
標題: | Biogenesis of C. elegans spermatogenesis small RNAs is initiated by a zc3h12a-like ribonuclease | 作者: | HSIN-YUE TSAI Cheng, Hsian-Tang Tsai, Yi-Ting |
關鍵字: | TRANSCRIPTOME-WIDE IDENTIFICATION; BINDING PROTEIN; GENE-EXPRESSION; SITES; KINASES; MEMORY; SPERM; DCR-1 | 公開日期: | 12-八月-2022 | 出版社: | AMER ASSOC ADVANCEMENT SCIENCE | 卷: | 8 | 期: | 32 | 來源出版物: | Science Advances | 摘要: | Small RNAs regulate spermatogenesis across species ranging from Caenorhabditis elegans to humans. In C. elegans, two Argonaute proteins, ALG-3 and ALG-4, and their associated alg-3/4 26G-small RNAs are essential for spermiogenesis at 25°C. The alg-3/4 26G-small RNAs are antisense to their target mRNAs and produced by the RNA-dependent RNA polymerase, RRF-3. However, it remains unclear how the RNA templates for RRF-3 are generated and which cellular processes are affected by alg-3/4 26G-small RNAs. Here, we demonstrate a previously unidentified zc3h12a-like ribonuclease protein, NYN-3, in alg-3/4 26G-small RNAs biogenesis. NYN-3 is not only required for proper abundance of alg-3/4 26G-small RNAs but also crosslinked to their targeted mRNAs before RRF-3 from ePAR-CLIP-seq. Bioinformatics analysis was then used to parse the 26G-small RNA-targeted genes into functional subclasses. Collectively, these findings implicate NYN-3 as an initiator of alg-3/4 26G-small RNA generation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/626973 | ISSN: | 2375-2548 | DOI: | 10.1126/sciadv.abm0699 |
顯示於: | 分子醫學研究所 |
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