https://scholars.lib.ntu.edu.tw/handle/123456789/627277
標題: | Comparison of DNA stabilizers and storage conditions on preserving fecal microbiota profiles | 作者: | CHIEH-CHANG CHEN WEI-KAI WU Chang, Chih-Min Panyod, Suraphan TZU-PIN LU JYH-MING LIOU YU-JEN FANG ERIC YAO-YU CHUANG MING-SHIANG WU |
關鍵字: | 16S rRNA amplicon sequencing; DNA stabilizers; Fecal microbiota; OMNIgene.GUT; Storage condition; Stratec | 公開日期: | 十二月-2020 | 出版社: | ELSEVIER TAIWAN | 卷: | 119 | 期: | 12 | 起(迄)頁: | 1791 | 來源出版物: | Journal of the Formosan Medical Association = Taiwan yi zhi | 摘要: | Appropriate storage of fecal samples is a critical step for the unbiased analysis of microbial communities in metagenomic studies. Rapid freezing at -80 °C is usually considered to be best practice, but this approach is challenging. DNA stabilizing kits may provide a more convenient method to preserve and store clinical samples. We evaluated the reliability of two collection kits (Stratec stool collection tube with stabilizer, #1038111200 and OMNIgene.GUT OMR-200) on preserving fecal microbiota. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627277 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85080052071&doi=10.1016%2fj.jfma.2020.01.013&partnerID=40&md5=bc76ad112448350b43812f44d6c35cf6 |
ISSN: | 0929-6646 | DOI: | 10.1016/j.jfma.2020.01.013 |
顯示於: | 醫學系 |
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