https://scholars.lib.ntu.edu.tw/handle/123456789/627480
標題: | Integrating high-throughput exposure assessment and in vitro screening data to prioritize endocrine-active potential and dietary risks of pesticides and veterinary drug residues in animal products | 作者: | YU-SYUAN LUO Chiu, Zi-Yi KUEN-YUH WU Hsu, Chao-Cheng |
關鍵字: | Chemical prioritization; Endocrine-active potential; High-throughput exposure modeling; High-throughput screening; Veterinary drugs | 公開日期: | 25-一月-2023 | 出版社: | Elsevier Ltd | 卷: | 173 | 來源出版物: | Food and Chemical Toxicology | 摘要: | New approach methodologies in toxicology, such as in vitro high-throughput screening (HTS), can minimize the use of experimental animals and allow mechanism-based predictions of in vivo toxicity. HTS data has been increasingly used in the regulatory context; however, only a few studies integrated dietary exposure and HTS data to foster chemical prioritization in food. Additionally, the endocrine-associated risk of veterinary drug residues in food is yet to be fully characterized. This study aims to systematically compare the translated HTS data with the acceptable daily intake (ADI) values and prioritize the pesticides and veterinary drug residues (n = 294) in food using the exposure-activity ratio (EAR) and Toxicological Prioritization index (ToxPi). The dietary exposure assessment was accomplished using a stochastic human exposure and dose simulation high-throughput model (SHEDS-HT). We selected 76 HTS assays from 12 nuclear receptors to represent the molecular initiating event (MIE) of endocrine-disrupting phenotypes. Chemical prioritization was achieved using 4 methods (i.e., EAR-OED, EAR-ADI, ToxPi-exposure + ADI, and ToxPi-exposure + endocrine score), where the consensus prioritized chemicals were fipronil, furazolidone, oxolinic acid, and oxytetracycline for the Taiwanese population. This case study demonstrates the utility of HTS data in fostering regulatory decisions on chemicals, especially for those lacking comprehensive toxicity data. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627480 | ISSN: | 0278-6915 | DOI: | 10.1016/j.fct.2023.113639 |
顯示於: | 食品安全與健康研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。