https://scholars.lib.ntu.edu.tw/handle/123456789/627590
標題: | Electrochemical Determination of Interaction between SARS-CoV-2 Spike Protein and Specific Antibodies | 作者: | Drobysh, Maryia Liustrovaite, Viktorija Baradoke, Ausra Rucinskiene, Alma Ramanaviciene, Almira Ratautaite, Vilma Viter, Roman CHIEN-FU CHEN Plikusiene, Ieva Samukaite-Bubniene, Urte Slibinskas, Rimantas Ciplys, Evaldas Simanavicius, Martynas Zvirbliene, Aurelija Kucinskaite-Kodze, Indre Ramanavicius, Arunas |
關鍵字: | COVID-19; SARS-CoV-2 coronavirus; antigen-antibody complex; cyclic voltammetry (CV); electrochemical immunosensor; electrochemical impedance spectroscopy (EIS); self-assembled monolayer (SAM); serological diagnosis; specific antibodies; spike proteins (rSpike) | 公開日期: | 17-六月-2022 | 出版社: | MDPI | 卷: | 23 | 期: | 12 | 來源出版物: | International journal of molecular sciences | 摘要: | The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627590 | ISSN: | 16616596 | DOI: | 10.3390/ijms23126768 |
顯示於: | 應用力學研究所 |
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