https://scholars.lib.ntu.edu.tw/handle/123456789/627591
標題: | Palladium-platinum bimetallic nanomaterials and their application in Staphylococcus aureus detection on paper-based devices | 作者: | Wu, Shou-Cheng Tsai, Tsung-Ting Li, Tsung-Hua Tung, Cheng-Yang Chiu, Ping-Yeh Lin, Jia-Hui CHIEN-FU CHEN |
關鍵字: | Paper-based analytical device; Pd@Pt nanoparticle; Point-of-care testing; Protein A; Staphylococcus aureus | 公開日期: | 15-十一月-2022 | 出版社: | ELSEVIER ADVANCED TECHNOLOGY | 卷: | 216 | 來源出版物: | Biosensors & bioelectronics | 摘要: | In this study, we demonstrate how palladium@platinum nanoparticles (Pd@Pt NPs) can be used as a nanozyme for the detection of Staphylococcus aureus (S. aureus) on a paper-based analytical device. Pt was doped with Pd to form bimetallic NPs that serve as a peroxidase mimetic, which can enhance the reaction area with a substrate (3, 3', 5, 5'-tetramethylbenzidine, TMB). After material characterization, we show the peroxidase-like activity of the Pd@Pt NPs featured a 13-times higher binding affinity value (Km) for TMB compared to horseradish peroxidase, which is currently widely used in immunoassays. By incorporating the Pd@Pt NPs in a paper-based immunoassay, we can detect protein A, the biomarker of S. aureus, within 30 min with a detection limit of 9.56 ng/mL. We have also successfully validated this nanozyme-immobilized paper-based analytical device (nPAD) for the detection of human immunoglobulin G to demonstrate the capability of the Pd@Pt NPs for different target analytes. This highly sensitive, rapid, and portable nPAD design has the potential for personalized medicine and point-of-care testing, which could expand on-site prognoses in resource-limited settings. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627591 | ISSN: | 0956-5663 | DOI: | 10.1016/j.bios.2022.114669 |
顯示於: | 應用力學研究所 |
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