https://scholars.lib.ntu.edu.tw/handle/123456789/627813
標題: | GALNT2 promotes invasiveness of colorectal cancer cells partly through AXL | 作者: | Liao, Ying-Yu Chuang, Ya-Ting Lin, Hsuan-Yu NENG-YU LIN Hsu, Tzu-Wen Hsieh, Szu-Chia Chen, Syue-Ting JI-SHIANG HUNG Yang, Hung-Jen JIN-TUNG LIANG MIN-CHUAN HUANG JOHN HUANG |
關鍵字: | AXL; GALNT; glycosylation; invasion | 公開日期: | 一月-2023 | 出版社: | WILEY | 卷: | 17 | 期: | 1 | 來源出版物: | Molecular oncology | 摘要: | GalNAc-type O-glycosylation and its initiating GalNAc transferases (GALNTs) play crucial roles in a wide range of cellular behaviors. Among 20 GALNT members, GALNT2 is consistently associated with poor survival of patients with colorectal cancer in public databases. However, its clinicopathological significance in colorectal cancer remains unclear. In this study, immunohistochemistry showed that GALNT2 was overexpressed in colorectal tumors compared with the adjacent nontumor tissues. GALNT2 overexpression was associated with poor survival of colorectal cancer patients. Forced expression of GALNT2 promoted migration and invasion as well as peritoneal metastasis of colorectal cancer cells. In contrast, GALNT2 knockdown with siRNAs or knockout with CRISPR/Cas9 system suppressed these malignant properties. Interestingly, we found that GALNT2 modified O-glycans on AXL and determined AXL levels via the proteasome-dependent pathway. In addition, the GALNT2-promoted invasiveness was significantly reversed by AXL siRNAs. These findings suggest that GALNT2 promotes colorectal cancer invasion at least partly through AXL. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627813 | ISSN: | 1574-7891 | DOI: | 10.1002/1878-0261.13347 |
顯示於: | 解剖學暨細胞生物學科所 |
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