https://scholars.lib.ntu.edu.tw/handle/123456789/628084
標題: | Screening and characterization of myositis-related autoantibodies in COVID-19 patients | 作者: | Teo, Kai-Fa Chen, Der-Yuan Hsu, Jeh-Ting Lai, Yi-Hua Chang, Ching-Kun PO-REN HSUEH Lan, Joung-Liang Hsu, Jye-Lin |
關鍵字: | GENE 5; JAPANESE PATIENTS; ANTIBODIES; ASSOCIATION; DERMATOMYOSITIS | 公開日期: | 一月-2023 | 出版社: | WILEY | 卷: | 16 | 期: | 1 | 來源出版物: | Clinical and translational science | 摘要: | An efficient host immune response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) appears to be crucial for controlling and resolving this viral infection. However, many studies have reported autoimmune characteristics in severe COVID-19 patients. Moreover, clinical observations have revealed that COVID-19-associated acute distress respiratory syndrome shares many features in common with inflammatory myopathy including interstitial lung disease (ILD), most particularly rapidly progressive (RP)-ILD. This study explored this phenomenon by seeking to identify and characterize myositis-specific and related autoantibodies in 25 COVID-19 patients with mild or severe symptoms. Line blot analysis with the EUROLINE Myopathies Ag kit identified 9 (36%) patients with COVID-19 with one or more autoantibodies against several myositis-related antigens (Jo-1, Ku, Mi-2β, PL-7, PL-12, PM-Scl 75, PM-Scl 100, Ro-52, and SRP); no anti-MDA5 antibodies were detected. As the presence of antibodies identified by line blots was unrelated to disease severity, we further characterized the autoantibodies by radioimmunoassay, in which [35 S]methionine-labeled K562 cellular antigens were precipitated and visualized by gel electrophoresis. This result was confirmed by an immunoprecipitation assay and immunoblotting; 2 patients exhibited anti-Ku70 and anti-Ku80 antibodies. Our data suggest that it is necessary to use more than one method to characterize and evaluate autoantibodies in people recovered from COVID-19, in order to avoid misinterpreting those autoantibodies as diagnostic markers for autoimmune diseases. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/628084 | ISSN: | 1752-8054 | DOI: | 10.1111/cts.13434 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。