https://scholars.lib.ntu.edu.tw/handle/123456789/629776
標題: | Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing | 作者: | Griffin, Donald R Archang, Maani M CHEN-HSIANG KUAN Weaver, Westbrook M Weinstein, Jason S Feng, An Chieh Ruccia, Amber Sideris, Elias Ragkousis, Vasileios Koh, Jaekyung Plikus, Maksim V Carlo, Dino Di Segura, Tatiana Scumpia, Philip O |
公開日期: | 30-五月-2020 | 來源出版物: | bioRxiv | 摘要: | Biomaterial scaffolds represent a promising approach for material-based tissue regeneration. We previously developed microporous annealed particle (MAP) hydrogels - a flowable, microparticle-based hydrogel in which neighboring hydrogel particles are linked in situ to form a porous scaffold that accelerates wound healing. To promote more extensive tissue ingrowth before scaffold degradation, we aimed to slow scaffold degradation by switching the chirality of the crosslinking peptides from L-peptides to D-peptides. Unexpectedly, despite showing the predicted slower enzymatic degradation in vitro, D-peptide crosslinked MAP hydrogel (D-MAP) hastened material degradation in vivo and imparted significant tissue regeneration to healed cutaneous wounds, including increased tensile strength and hair neogenesis. By themselves, D-chiral peptides were poor activators of macrophage innate immune signaling in vivo, but MAP particles elicit IL-33 type 2 myeloid cell recruitment which is amplified in vivo in the presence of D-peptides. Remarkably, D-MAP elicited significant antigen-specific immunity against the D-chiral peptides, and an intact adaptive immune system was required for the hydrogel-induced skin regeneration. These findings demonstrate that the generation of an adaptive immune response from a biomaterial is sufficient to induce cutaneous regenerative healing despite faster scaffold degradation. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/629776 | ISSN: | 26928205 | DOI: | 10.1101/2020.05.27.117317 |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。