https://scholars.lib.ntu.edu.tw/handle/123456789/630243
標題: | Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel-induced expression for vascular endothelial growth factor in HepG2 cells | 作者: | Lin, Jau Tien Chang, Yuan Yen YI-CHEN CHEN Liao, Po Lin Yang, Deng Jye |
關鍵字: | HepG2 cell; hypoxia inducible factor (HIF)-1α; litchi flower; mechanism; nickel (Ni); proanthocyanidin; vascular endothelial growth factor (VEGF) | 公開日期: | 1-七月-2019 | 出版社: | WILEY | 卷: | 43 | 期: | 7 | 來源出版物: | Journal of Food Biochemistry | 摘要: | The protective efficacy of litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction (LFPF) composed of (-)-epicatechin and proanthocyanidin A2 against vascular endothelial growth factor (VEGF) generation induced by nickel (Ni) in hepatocellular carcinoma (Hep G2) cells was studied. VEGF is an angiogenic inducer, which promotes tumor angiogenesis, leading to rapid tumor growth and metastasis. VEGF could be substantially induced in the Ni-mediated Hep G2 cells. Through LFPF treatment, the Ni-induced VEGF generation could be suppressed significantly. The inhibition of HIF-1α expression by blocking phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways, and the suppression of Janus kinase 2 (JAK2)/signal transducers and activators of transcription 3 (STAT 3), and Raf-1 proto-oncogene, serine/threonine kinase (RAF1)/mitogen-activated protein kinase (MEK1/2)/extracellular-signal-regulated kinase (ERK1/2) pathways are important molecular mechanisms for the LFPF action. LFPF should probably reduce the risk of liver cancer in Ni-contaminated environments by inhibiting VEGF expression. Practical applications: LFPF mainly contained (-)-epicatechin and proanthocyanidin A2. Our results demonstrated that LFPF considerably suppressed the Ni-induced VEGF expression through inhibition of JAK2/STAT 3 and RAF1/MEK1/2/ERK1/2 pathways and prohibited HIF-1α expression through blocking PI3K/AKT/mTOR pathway. Litchi flowers might have the potential to diminish the liver cancer risk in a Ni-contaminated environment through suitable treatment. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065241511&doi=10.1111%2fjfbc.12882&partnerID=40&md5=0b10e44aaf927d94af32ba7f4952c3c1 https://scholars.lib.ntu.edu.tw/handle/123456789/630243 |
ISSN: | 01458884 | DOI: | 10.1111/jfbc.12882 |
顯示於: | 動物科學技術學系 |
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