https://scholars.lib.ntu.edu.tw/handle/123456789/630426
標題: | Analysis of gut microbiota and the effect of lauric acid against necrotic enteritis in Clostridium perfringens and Eimeria side-by-side challenge model | 作者: | WEN-YUAN YANG YUE-JIA LEE Lu, Hsinyi CHUNG-HSI CHOU Wang, Chinling |
關鍵字: | CHAIN FATTY-ACIDS; CAMPYLOBACTER-JEJUNI COLONIZATION; BACTERIAL METABOLIC PRODUCTS; BROILER-CHICKENS; PREDISPOSING FACTORS; TRACT; ENTEROCOLITIS; TRIGLYCERIDES; PATHOGENESIS; COCCIDIOSIS | 公開日期: | 1-五月-2019 | 出版社: | PUBLIC LIBRARY SCIENCE | 卷: | 14 | 期: | 5 | 來源出版物: | PLoS ONE | 摘要: | Gut microbiota has been demonstrated to be involved in intestinal nutrition, defense, and immunity, as well as participating in disease progression. This study was to investigate gut microbiota changes in chickens challenged with netB-positive Clostridium perfringens strain (CP1) and/or the predisposing Eimeria species (Eimeria) and fed diets with fishmeal supplementation. In addition, the effects of lauric acid, a medium-chain fatty acid (MCFA), on necrotic enteritis (NE) reduction and modulation of microbiota were evaluated. The results demonstrated that microbial communities in the jejunum were distinct from those in the cecum, and the microbial community change was more significant in jejunum. Challenge of CP1 in conjunction with Eimeria significantly reduced species diversity in jejunal microbiota, but cecal microbiota remained stable. In the jejunum, CP1 challenge increased the abundance of the genera of Clostridium sensu stricto 1, Escherichia Shigella, and Weissella, but significantly decreased the population of Lactobacillus. Eimeria infection on its own was unable to promote NE, demonstrating decrements of Clostridium sensu stricto 1 and Lactobacillus. Co-infection with CP1 and Eimeria reproduced the majority of NE lesions with significant increment of Clostridium sensu stricto 1 and reduction in Lactobacillus. The advance of changes on these two taxa increased the severity of NE lesions. Further analyses of metagenomeSeq, STAMP, and LEfSe consistently showed significant overgrowth of Clostridium sensu stricto 1 was associated with NE. The supplementation of lauric acid did not reduce NE incidence and severity but decreased the relative abundance of Escherichia Shigella. In conclusion, significant overgrowth of C. perfringens as well as other Clostridium species in Clostridium sensu stricto 1 with the decrement of Lactobacillus in the jejunum is the featured microbiota correlated with NE. Controlling proliferation of Clostridium sensu stricto 1 and manipulation of Lactobacillus in the jejunum should be the strategy to prevent NE. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066437823&doi=10.1371%2fjournal.pone.0205784&partnerID=40&md5=6d776eea035c1208cd38443f29d9859d https://scholars.lib.ntu.edu.tw/handle/123456789/630426 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0205784 |
顯示於: | 獸醫學系 |
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