https://scholars.lib.ntu.edu.tw/handle/123456789/630434
標題: | Prevalence and persistence of Listeria monocytogenes in ready-to-eat tilapia sashimi processing plants | 作者: | Chen, Bang Yuan Wang, Chung Yi Wang, Chia Lan Fan, Yang Chi Weng, I. Ting CHUNG-HSI CHOU |
關鍵字: | Listeria monocytogenes; Pulsed-field gel electrophoresis; Ready to eat; Sashimi; Tilapia | 公開日期: | 1-十一月-2016 | 出版社: | INT ASSOC FOOD PROTECTION | 卷: | 79 | 期: | 11 | 起(迄)頁: | 1898 | 來源出版物: | Journal of Food Protection | 摘要: | A 2-year study was performed at two ready-to-eat tilapia sashimi processing plants (A and B) to identify possible routes of contamination with Listeria monocytogenes during processing. Samples were collected from the aquaculture environments, transportation tanks, processing plants, and final products. Seventy-nine L. monocytogenes isolates were found in the processing environments and final products; 3.96% (50 of 1,264 samples) and 3.86% (29 of 752 samples) of the samples from plants A and B, respectively, were positive for L. monocytogenes. No L. monocytogenes was detected in the aquaculture environments or transportation tanks. The predominant L. monocytogenes serotypes were 1/2b (55.70%) and 4b (37.97%); serotypes 3b and 4e were detected at much lower percentages. At both plants, most processing sections were contaminated with L. monocytogenes before the start of processing, which indicated that the cleaning and sanitizing methods did not achieve adequate pathogen removal. Eleven seropulsotypes were revealed by pulsed-field gel electrophoresis and serotyping. Analysis of seropulsotype distribution revealed that the contamination was disseminated by the processing work; the same seropulsotypes were repeatedly found along the work flow line and in the final products. Specific seropulsotypes were persistently found during different sampling periods, which suggests that the sanitation procedures or equipment used at these plants were inadequate. Plant staff should improve the sanitation procedures and equipment to reduce the risk of L. monocytogenes cross-contamination and ensure the safety of ready-to-eat tilapia products. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992738310&doi=10.4315%2f0362-028X.JFP-16-149&partnerID=40&md5=dabd7fc9c5ece9a556baa9fd8e2a5652 https://scholars.lib.ntu.edu.tw/handle/123456789/630434 |
ISSN: | 0362028X | DOI: | 10.4315/0362-028X.JFP-16-149 | SDG/關鍵字: | aquaculture; cleaning; contamination; human; Listeria monocytogenes; major clinical study; nonhuman; prevalence; pulsed field gel electrophoresis; safety; sampling; serotype; serotyping; staff; Tilapia; workflow; animal; classification; food contamination; food control; food industry; Listeria monocytogenes; prevalence; pulsed field gel electrophoresis |
顯示於: | 獸醫學系 |
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