https://scholars.lib.ntu.edu.tw/handle/123456789/630495
標題: | Overexpression of chemokine ligand 7 is associated with the progression of canine transmissible venereal tumor | 作者: | Chiang, Hsin Chien Wang, Yu Shan CHUNG-HSI CHOU TAI-CHING LIAO Chu, Rea Min CHEN-SI LIN |
關鍵字: | CTVT; CXCL7; CXCR2; IL-6; TGF-β | 公開日期: | 9-十一月-2012 | 出版社: | BIOMED CENTRAL LTD | 卷: | 8 | 期: | 1 | 來源出版物: | BMC Veterinary Research | 摘要: | Background: Chemokines play multiple roles in the development and progression in a variety of tumors. Chemokine (C-X-C motif) ligand 7 (CXCL7) has been found associated with pro-inflammatory responses, but its role in cancer growth remains unclear. Our previous study showed that R phase tumor infiltrating lymphocytes (TILs) produced large amounts of interleukin (IL)-6 which antagonized transforming growth factor (TGF)-β derived from CTVT to diminish the immune-suppressive microenvironment. Now we intend to determine the expression pattern of CXCL7 and the role of IL-6/TGF-β in CXCL7 induction during spontaneous progressive (P) and regressive (R) phases in canine transmissible venereal tumor (CTVT).Results: We have demonstrated that CXCL7 expressed at high level in P phase and down-regulated in R phase by western blot and real-time PCR. This suggested that CXCL7 expression was negatively correlated with the tumor growth. Co-culturing TILs with CTVT cells was found to reduce CXCL7 expression, while adding IL-6 blocking antibody reversed it. Moreover, in P phase CTVT, while IL-1β and TGF-β had no obvious effect on CXCL7 expression, IL-6 was found significantly to reduce CXCL7 expression in a dose-dependent manner. The mRNA expression results of CXCL7 receptor, CXCR2, further confirmed the effects of IL-6 concentration on the CXCL7 expression.Conclusion: CXCL7 overexpression might be associated with the progressive growth of CTVT. The results shown here also suggest the role of CXCL7 in cancer development and the potential as the anti-cancer therapeutic target. © 2012 Chiang et al.; licensee BioMed Central Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84868544948&doi=10.1186%2f1746-6148-8-216&partnerID=40&md5=253cddeab02b92690c402ca261e09b63 https://scholars.lib.ntu.edu.tw/handle/123456789/630495 |
ISSN: | 1746-6148 | DOI: | 10.1186/1746-6148-8-216 | SDG/關鍵字: | animal; animal venereal tumor; article; cell culture; dog; dog disease; gene expression regulation; genetics; male; metabolism; physiology |
顯示於: | 獸醫學系 |
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