https://scholars.lib.ntu.edu.tw/handle/123456789/630833
標題: | Brown Algae-Derived Fucoidan Exerts Oxidative Stress-Dependent Antiproliferation on Oral Cancer Cells | 作者: | Shiau, Jun-Ping Chuang, Ya-Ting Yang, Kun-Han Chang, Fang-Rong Sheu, Jyh-Horng Hou, Ming-Feng JIIANG-HUEI JENG Tang, Jen-Yang Chang, Hsueh-Wei |
關鍵字: | algae; dietary natural product; fucoidan; oral cancer; oxidative stress; polysaccharide | 公開日期: | 26-四月-2022 | 出版社: | MDPI | 卷: | 11 | 期: | 5 | 來源出版物: | Antioxidants (Basel, Switzerland) | 摘要: | Fucoidan is a dietary brown algae-derived fucose-rich polysaccharide. However, the anticancer effects of fucoidan for oral cancer treatment remain unclear, particularly in terms of its preferential antiproliferation ability and oxidative-stress-associated responses. This study first evaluated the effects and mechanisms of the preferential antiproliferation of fucoidan between oral cancer and non-malignant oral cells (S-G). In a 48 h MTS assay, fucoidan showed higher antiproliferation in response to five types of oral cancer cells, but not S-G cells, demonstrating preferential antiproliferation of oral cancer cells. Oral cancer cells (Ca9-22 and CAL 27) showing high sensitivity to fucoidan were selected to explore the antiproliferation mechanism compared to S-G cells. Fucoidan showed subG1 accumulation and an annexin V increase in apoptosis, accompanied by caspase 8, 9, and 3 activations in oral cancer cells, but not in S-G cells. Fucoidan increased reactive oxygen species and mitochondrial superoxide levels and decreased cellular glutathione in oral cancer cells compared with S-G cells. These oxidative stress effects were attributed to the downregulation of antioxidant signaling genes (NRF2, TXN, and HMOX1) in oral cancer cells rather than S-G cells. Fucoidan showed DNA damage-inducible effects (γH2AX and 8-hydroxy-2-deoxyguanosine) in oral cancer cells but not in S-G cells. Accordingly, these preferential changes in oral cancer but not in non-malignant cells contribute to the preferential antiproliferation mechanism of fucoidan. Furthermore, these changes were reverted by pretreatment with the antioxidant N-acetylcysteine. Therefore, for the first time, this study provides a detailed understanding of the preferential antiproliferation effects and mechanisms of fucoidan in oral cancer cells. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630833 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox11050841 |
顯示於: | 臨床牙醫學研究所 |
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