https://scholars.lib.ntu.edu.tw/handle/123456789/630839
標題: | Fucoidan/UVC Combined Treatment Exerts Preferential Antiproliferation in Oral Cancer Cells but Not Normal Cells | 作者: | Chuang, Ya-Ting Shiau, Jun-Ping Yen, Ching-Yu Hou, Ming-Feng JIIANG-HUEI JENG Tang, Jen-Yang Chang, Hsueh-Wei |
關鍵字: | combined treatment; fucoidan; oral cancer; oxidative stress; ultraviolet C | 公開日期: | 12-九月-2022 | 出版社: | MDPI | 卷: | 11 | 期: | 9 | 來源出版物: | Antioxidants (Basel, Switzerland) | 摘要: | Combined treatment is a promising anticancer strategy for improving antiproliferation compared with a single treatment but is limited by adverse side effects on normal cells. Fucoidan (FN), a brown-algae-derived polysaccharide safe food ingredient, exhibits preferential function for antiproliferation to oral cancer but not normal cells. Utilizing the preferential antiproliferation, the impacts of FN in regulating ultraviolet C (UVC) irradiation were assessed in oral cancer cells. A combined treatment (UVC/FN) reduced cell viability of oral cancer cells (Ca9-22 and CAL 27) more than single treatments (FN or UVC), i.e., 53.7%/54.6% vs. 71.2%/91.6%, and 89.2%/79.4%, respectively, while the cell viability of UVC/FN treating on non-malignant oral (S-G) was higher than oral cancer cells, ranging from 106.0 to 108.5%. Mechanistically, UVC/FN preferentially generated higher subG1 accumulation and apoptosis-related inductions (annexin V, caspases 3, 8, and 9) in oral cancer cells than single treatments. UVC/FN preferentially generated higher oxidative stress than single treatments, as evidenced by flow cytometry-detecting reactive oxygen species, mitochondrial superoxide, and glutathione. Moreover, UVC/FN preferentially caused more DNA damage (γH2AX and 8-hydroxy-2'-deoxyguanosine) in oral cancer cells than in single treatments. N-acetylcysteine pretreatment validated the oxidative stress effects in these UVC/FN-induced changes. Taken together, FN effectively enhances UVC-triggered antiproliferation to oral cancer cells. UVC/FN provides a promising potential for preferential and synergistic antiproliferation in antioral cancer therapy. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630839 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox11091797 |
顯示於: | 臨床牙醫學研究所 |
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