https://scholars.lib.ntu.edu.tw/handle/123456789/631241
標題: | Diagonal Interactions between Glutamate and Arginine Analogs with Varying Side-Chain Lengths in a β-Hairpin | 作者: | Li, Nian-Zhi Yu, Chen-Hsu Wu, Jhuan-Yu Huang, Shing-Jong Huang, Shou-Ling RICHARD PING CHENG |
關鍵字: | charged amino acid; diagonal interaction; ion-pairing interaction; peptide; side-chain length; β-hairpin | 公開日期: | 23-三月-2023 | 出版社: | MDPI | 卷: | 28 | 期: | 7 | 來源出版物: | Molecules (Basel, Switzerland) | 摘要: | Cross-strand interactions are important for the stability of β-sheet structures. Accordingly, cross-strand diagonal interactions between glutamate and arginine analogs with varying side-chain lengths were studied in a series of β-hairpin peptides. The peptides were analyzed by homonuclear two-dimensional nuclear magnetic resonance methods. The fraction folded population and folding free energy of the peptides were derived from the chemical shift data. The fraction folded population trends could be rationalized using the strand propensity of the constituting residues, which was not the case for the peptides with lysine analogs, highlighting the difference between the arginine analogs and lysine analogs. Double-mutant cycle analysis was used to derive the diagonal ion-pairing interaction energetics. The most stabilizing diagonal cross-strand interaction was between the shortest residues (i.e., Asp2-Agp9), most likely due to the least side-chain conformational penalty for ion-pair formation. The diagonal interaction energetics in this study involving the arginine analogs appears to be consistent with and extend beyond our understanding of diagonal ion-pairing interactions involving lysine analogs. The results should be useful for designing β-strand-containing molecules to affect biological processes such as amyloid formation and protein-protein interactions. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631241 | ISSN: | 1420-3049 | DOI: | 10.3390/molecules28072888 |
顯示於: | 化學系 |
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