https://scholars.lib.ntu.edu.tw/handle/123456789/631958
標題: | Simultaneous toxicokinetic studies of aristolochic acid I and II and aristolactam I and II using a newly-developed microdialysis liquid chromatography-tandem mass spectrometry | 作者: | Chiang, Su-Yin Wey, Ming-Tsai YU-SYUAN LUO Shih, Wei-Chung Chimeddulam, Dalaijamts Hsu, Po-Chi Huang, Hui-Fen Tsai, Tung-Hu KUEN-YUH WU |
關鍵字: | Aristolactam; Aristolochic acid; Balkan endemic nephropathy; In vivo microdialysis; Liquid chromatography-tandem mass spectrometry; Toxicokinetics | 公開日期: | 29-五月-2023 | 出版社: | Elsevier Science Ltd | 卷: | 177 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Aristolochic acids (AAs) are naturally occurring genotoxic carcinogens linked to Balkan endemic nephropathy and aristolochic acid nephropathy. Aristolochic acid I and II (AA-I and AA-II) are the most abundant AAs, and AA-I has been reported to be more genotoxic and nephrotoxic than AA-II. The study aimed to explore metabolic differences underlying the differential toxicity. We developed a novel microdialysis sampling coupled with solid-phase extraction liquid chromatography-tandem mass spectrometry (MD-SPE-LC-MS/MS) to simultaneously study the toxicokinetics (TK) of AA-I and AA-II and their corresponding aristolactams (AL-I and AL-II) in the blood of Sprague Dawley rats co-treated with AA-1 and AA-II. Near real-time monitoring of these analytes in the blood of treated rats revealed that AA-I was absorbed, distributed, and eliminated more rapidly than AA-II. Moreover, the metabolism efficiency of AA-I to AL-I was higher compared to AA-II to AL-II. Only 0.58% of AA-I and 0.084% of AA-II was reduced to AL-I and AL-II, respectively. The findings are consistent with previous studies and support the contention that differences in the in vivo metabolism of AA-I and AA-II may be critical factors for their differential toxicities. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/631958 | ISSN: | 02786915 | DOI: | 10.1016/j.fct.2023.113856 |
顯示於: | 食品安全與健康研究所 |
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