https://scholars.lib.ntu.edu.tw/handle/123456789/632641
標題: | Monitoring Cultured Rat Hepatocytes Using RNA-Seq In Vitro | 作者: | YUNG-TE HOU Wu, Chia Chun Wang, Wen Ting Yang, Wen Tse Liao, Ying Hsiu CHIEN-YU CHEN |
關鍵字: | hepatocyte culture; qPCR; RNA-Seq | 公開日期: | 1-四月-2023 | 出版社: | MDPI | 卷: | 24 | 期: | 8 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Compared to other techniques, RNA sequencing (RNA-Seq) has the advantage of having details of the expression abundance of all transcripts in a single run. In this study, we used RNA-Seq to monitor the maturity and dynamic characteristics of in vitro hepatocyte cultures. Hepatocytes, including mature hepatocytes and small hepatocytes, were analyzed in vitro using RNA-Seq and quantitative polymerase chain reaction (qPCR). The results demonstrated that the gene expression profiles measured by RNA-Seq showed a similar trend to the expression profiles measured by qPCR, and can be used to infer the success of in vitro hepatocyte cultures. The results of the differential analysis, which compared mature hepatocytes against small hepatocytes, revealed 836 downregulated and 137 upregulated genes. In addition, the success of the hepatocyte cultures could be explained by the gene list screened from the adopted gene enrichment test. In summary, we demonstrated that RNA-Seq could become an effective method for monitoring the whole transcriptome of hepatocyte cultures and provide a more comprehensive list of factors related to the differentiation of small hepatocytes into mature hepatocytes. This monitoring system not only shows high potential in medical applications but may also be a novel method for the clinical diagnosis of liver-related diseases. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85156181854&doi=10.3390%2fijms24087534&partnerID=40&md5=96d8136aae5d54b79501f7d4cd83cc86 https://scholars.lib.ntu.edu.tw/handle/123456789/632641 |
ISSN: | 16616596 | DOI: | 10.3390/ijms24087534 |
顯示於: | 生物機電工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。