https://scholars.lib.ntu.edu.tw/handle/123456789/633128
標題: | Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging | 作者: | SUNG-JAN LIN SHIOU-HWA JEE Kuo, Chien Jui Wu, Ruei Jr WEI-CHOU LIN JAU-SHIUH CHEN YI-HUA LIAO Hsu, Chih Jung TSEN-FANG TSAI YANG-FANG CHEN CHEN-YUAN DONG |
公開日期: | 15-九月-2006 | 卷: | 31 | 期: | 18 | 來源出版物: | Optics Letters | 摘要: | We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsiblefor SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs’ surgery in the real-time guidance of the secure removal of basal cell carcinoma. © 2006 Optical Society of America. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633128 | ISSN: | 01469592 | DOI: | 10.1364/OL.31.002756 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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