https://scholars.lib.ntu.edu.tw/handle/123456789/633322
標題: | Modification of magnetite-doped NH2-MIL-100(Fe) with aliphatic C8 carbon chain for feasible protein purification in reversed-phase mode | 作者: | Hsu, Chung Hui Yu, Yu Sheng Gu, Yesong KEVIN CHIA-WEN WU |
關鍵字: | aliphatic C8 carbon chain | Magnetic Metal-Organic Framework composite | Protein separation and purification | Reversed-phase mode | 公開日期: | 1-五月-2022 | 卷: | 288 | 來源出版物: | Separation and Purification Technology | 摘要: | NH2-MIL-100(Fe) is a stable Metal-Organic Frameworks (MOFs) possessing numerous tunable properties. In this study, we have successfully modified NH2-MIL-100(Fe) with aliphatic C8 carbon chain to create a hydrophobic surface distribution. The detailed characterizations of NH2-MIL-100(Fe)-C8 were performed to demonstrate its attractive properties, such as unique MOF architecture, sufficient surface area, and surface hydrophobicity. In addition, the NH2-MIL-100(Fe)-C8 exhibited the distinctive hydrophobic interaction with an individual protein under a specific concentration of acetonitrile, and was able to separate the protein from a mixture with a good resolution. It has a binding capacity of 6.25 μg of BSA/mg particle. Since we have introduced Fe3O4 to NH2-MIL-100(Fe)-C8 to provide with excellent magnetization, the target protein could be easily collected by a magnet and the NH2-MIL-100(Fe)-C8 could be regenerated over 15 times without significant loss of its capacity. Therefore, NH2-MIL-100(Fe)-C8 provides researchers with a convenient and affordable tool for protein separation and purification in reversed-phase mode. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633322 | ISSN: | 13835866 | DOI: | 10.1016/j.seppur.2022.120528 |
顯示於: | 化學工程學系 |
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