https://scholars.lib.ntu.edu.tw/handle/123456789/634103
標題: | Fibrous polypeptide based bioscaffold delivery of minocycline hydrochloride for nerve regeneration | 作者: | Su, An Jey A. Jiang, Ning SHYH-CHYANG LUO Washington, Kia M. Wu, Ming Chung Huang, Yu Ching WEI-FANG SU |
關鍵字: | Drug delivery | Glutamic acid | Minocycline hydrochloride | Polypeptides | Scaffold | 公開日期: | 1-九月-2023 | 卷: | 305 | 來源出版物: | Materials Chemistry and Physics | 摘要: | Neural tissue engineering is a technology with the potential to treat irreversible neurodegenerative diseases. We chose poly (γ-benzyl-L-glutamate) (PBG) and its hydrolyzed copolymer (poly (γ-benzyl-glutamate)80-r-(γ –glutamic acid)20) (PBGA) as the raw materials to make fibrous scaffolds by electrospinning process. These raw materials are biocompatible polypeptides which contain the neurotransmitter glutamate. Morphologically, the scaffolds consist of aligned fibers, which is important for directionality. Minocycline hydrochloride (MH) is a neuroprotective antibiotic obviously shown to enhance neurite growth, but is unstable in phosphate buffer solution. By incorporating and immobilizing MH into either PBG or PBGA polypeptide scaffolds, we created a stable drug delivery system. After 7-day release, the cumulative release of MH from PBGA scaffold was 42.65%. Nerve growth factor (NGF) responsive rat pheochromocytoma (PC12) cells were cultured on the scaffold to assess cell biocompatibility and proliferation. We found cell viability and differentiation improved when cultured on scaffolds bearing 2 wt% and 4 wt% of MH. PC12 cells cultured on PBGA incorporating 4 wt% MH exhibited the longest neurite outgrowth of 190.29 μm, the average is 33% increase over that of pristine PBGA after 5-days of NGF mediated differentiation. These polypeptide-based scaffolds loaded with MH are a promising drug delivery system for neural tissue engineering. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/634103 | ISSN: | 02540584 | DOI: | 10.1016/j.matchemphys.2023.127974 |
顯示於: | 材料科學與工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。