https://scholars.lib.ntu.edu.tw/handle/123456789/634753
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Pai, Yun Ling | en_US |
dc.contributor.author | Wu, Yueh Feng | en_US |
dc.contributor.author | SUNG-JAN LIN | en_US |
dc.date.accessioned | 2023-08-25T09:40:07Z | - |
dc.date.available | 2023-08-25T09:40:07Z | - |
dc.date.issued | 2022-11-10 | - |
dc.identifier.isbn | 9781450397223 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/634753 | - |
dc.description.abstract | The transparent corneal stroma, which consists of regularly packed collagen fibrils and keratocytes, comprises 90% of the corneal thickness. Injury response in corneal stroma compromises corneal transparency due to overproduction of abnormal extracellular matrix by keratocyte-myofibroblast transformation. Corneal transplantation is considered the optimal solution to restore vision. Whether the keratocyte-myofibroblast transformation can be reversed remains unclear. In this study, we first developed a mouse corneal mechanical injury model. At 24h after the injury, α-SMA expression significantly increased, and SHG signals changed with respect to the collagen orientation. In addition, we isolated primary human keratocytes in advance; thereafter, we can decipher the keratocytes-myofibroblast transformation by adding TGF-β1 in the future. This proposal established three platforms, including a mouse injury model, primary human cell culture, and transgenic mice for targeting keratocytes. We will integrate these systems with single-cell transcriptomics, ATAC-sequencing, and proteomics to comprehensively explore keratocyte-myofibroblast transformation, and eventually, identify therapeutic targets for treating corneal fibrosis. | en_US |
dc.relation.ispartof | ACM International Conference Proceeding Series | en_US |
dc.subject | Corneal opacification | fibrosis | keratocyte | myofibroblast | scar | wound healing | en_US |
dc.title | Integrative Analysis of Myofibroblast Transformation During Corneal Fibrosis | en_US |
dc.type | conference paper | en_US |
dc.identifier.doi | 10.1145/3574198.3574235 | - |
dc.identifier.scopus | 2-s2.0-85150360253 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85150360253 | - |
dc.relation.pageend | 241 | en_US |
item.openairetype | conference paper | - |
item.openairecristype | http://purl.org/coar/resource_type/c_5794 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Biomedical Engineering | - |
crisitem.author.dept | Dermatology | - |
crisitem.author.dept | Dermatology-NTUH | - |
crisitem.author.dept | Medical Research | - |
crisitem.author.dept | Center for Frontier Medicine | - |
crisitem.author.orcid | 0000-0003-1325-3464 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學工程學研究所 |
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