https://scholars.lib.ntu.edu.tw/handle/123456789/635258
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHIA-LUN CHAO | en_US |
dc.contributor.author | Chuang, Chun-Pin | en_US |
dc.contributor.author | Cheng, Yen-Fen | en_US |
dc.contributor.author | Lee, Kueir-Rarn | en_US |
dc.contributor.author | Chang, Yung | en_US |
dc.contributor.author | Cheng, Shun-Ping | en_US |
dc.contributor.author | Chan, Wan-Khey | en_US |
dc.contributor.author | Ho, Feng-Ming | en_US |
dc.date.accessioned | 2023-09-08T05:53:21Z | - |
dc.date.available | 2023-09-08T05:53:21Z | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 03603997 | - |
dc.identifier.uri | https://www.scopus.com/record/display.uri?eid=2-s2.0-84957583360&origin=inward&txGid=4583170f71c25ebf4c4cbe41c08a7c67 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/635258 | - |
dc.description.abstract | Diabetes mellitus may cause vascular endothelial damage via endothelial matrix metalloproteinase-2 (MMP-2). The role of endothelial autophagy in MMP-2-mediated cell injury in response to high-glucose (HG) stimulation was rarely described. In this study, we used HG-treated human umbilical vein endothelial cells (HUVECs) to investigate the effect of autophagy on MMP-2-induced cell transmigration and apoptosis. THP-1 transmigration was detected by the transmigration assay. Light chain 3 (LC3, representing autophagy), MMP-2, and poly (ADP-ribose) polymerase (PARP, representing apoptosis) of HG (33 mM)-treated HUVECs were evaluated by western blot analysis. The MMP-2 activity was also examined by gelatin zymography. We used GM6001 (10 μM, an MMP-2 inhibitor) to investigate the relationship of MMP-2 and THP-1 transmigration. Using 3-methyladenine (3MA, 5 mM, an LC3 inhibitor), we explored the effects of autophagy on MMP-2 expression, THP-1 transmigration, and apoptosis. Our results showed that HG increased LC3-II expression, MMP-2 activity, THP-1 transmigration, and cleaved PARP expression in a time-dependent manner (0-48 h); among them, LC3-II appeared earlier (0-24 h) than the others (24-48 h). GM6001 suppressed MMP-2 activity and ameliorated THP-1 transmigration. 3MA suppressed LC3-II expression and increased MMP-2 expression, THP-1 transmigration, and cleaved PARP expression. From these sequential findings, we demonstrated that autophagy plays a protective role in MMP-2-mediated cell transmigration and cell death in HG-stimulated HUVECs. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Inflammation | en_US |
dc.subject | autophagy; endothelial cells; high glucose; matrix metalloproteinase; transmigration | en_US |
dc.title | The Protective Role of Autophagy in Matrix Metalloproteinase-Mediated Cell Transmigration and Cell Death in High-Glucose-Treated Endothelial Cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1007/s10753-016-0313-7 | - |
dc.identifier.pmid | 26846884 | - |
dc.identifier.scopus | 2-s2.0-84957583360 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84957583360 | - |
dc.relation.pages | 830 | en_US |
dc.relation.journalvolume | 39 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pageend | 838 | en_US |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUHHC | - |
crisitem.author.orcid | 0000-0002-2804-7447 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
顯示於: | 醫學系 |
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