https://scholars.lib.ntu.edu.tw/handle/123456789/637121
標題: | Regulatory roles of microRNA163 in responses to stresses in Arabidopsis | 作者: | Kok, Zhenyuan Kuo, Yun-Wei Soh, Zhi Thong Huang, Hao-Chen Tseng, Bo-Shun Hsieh, Hung-Cheng Tsai, Wei-An SHIH-TONG JENG Chen, Shi-Peng Lin, Jeng-Shane |
公開日期: | 24-十月-2023 | 出版社: | John Wiley and Sons Inc | 卷: | 175 | 期: | 5 | 起(迄)頁: | Article number e14053 | 來源出版物: | Physiologia plantarum | 摘要: | MicroRNAs (miRNAs) are small regulatory RNAs that participate in various biological processes by silencing target genes. In Arabidopsis, microRNA163 (miR163) was found to be involved in seed germination, root development, and biotic resistance. However, the regulatory roles of miR163 remain unclear. In the current study, the mir163 mutant was investigated to comprehensively understand and characterize its functions in Arabidopsis. RNA-sequencing and Gene Ontology enrichment analyses revealed that miR163 might be involved in "response to stimulus" and "metabolic process". Interestingly, "response to stress", including heat, cold, and oxidative stress, was enriched under the subcategory of "response to stimulus". We observed that miR163 and PXMT were repressed and induced under heat stress, respectively. Furthermore, the study detected significant differences in seed germination rate, hypocotyl length, and survival rate, indicating a variation in the thermotolerance between WT and mir163 mutant. The results revealed that the mir163 mutant had a lesser degree of germination inhibition by heat treatment than WT. In addition, the mir163 mutant showed a better survival rate and longer hypocotyl length under heat treatment than the WT. The metabolomes of WT and mir163 mutant were further analyzed. The contents of benzene derivatives and flavonoids were affected by miR163, which could enhance plants' defense abilities. In conclusion, miR163/targets regulated the expression of stress-responsive genes and the accumulation of defense-related metabolites to alter stress tolerance. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637121 | ISSN: | 00319317 | DOI: | 10.1111/ppl.14053 |
顯示於: | 植物科學研究所 |
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