https://scholars.lib.ntu.edu.tw/handle/123456789/637264
標題: | Multiomics Strategy Reveals the Mechanism of Action and Ameliorating Effect of Deer Velvet Antler Water Extracts on DSS-Induced Colitis | 作者: | Hung, Ying Kai Ho, Shang Tse Kuo, Ching Yun MING-JU CHEN |
關鍵字: | inflammatory bowel disease | inflammatory response | intestinal barrier integrity | microbiota | velvet antler | 公開日期: | 1-七月-2023 | 卷: | 11 | 期: | 7 | 來源出版物: | Biomedicines | 摘要: | Velvet antler is a precious traditional Chinese medicine used for thousands of years. This study investigated the anti-colitis effects of water extracts of Formosan sambar deer (SVAE) and red deer (RVAE) to identify the possible mechanisms and the bioactive compounds using a dextran sulfate sodium (DSS)-induced colitis mouse model. The mechanism of action and the ameliorating effects of SVAE and RVAE on DSS-induced colitis were evaluated using a mouse model. Ultra-high performance liquid chromatography-mass/mass and gas chromatography-mass/mass were applied to identify the bioactive components of the SVAE and RVAE water extracts. The results revealed that both high-dose SVAE and RVAE could ameliorate the symptoms of colitis due to reduced systemic inflammatory responses, enhanced intestinal barrier integrity by restoration of tight junction proteins, and improved gut dysbiosis. The potentially bioactive components of SVAE and RVAE were identified as small molecules (<3 kDa). Further identification by untargeted metabolomics analysis suggested that l-carnitine, hypoxanthine, adrenic acid, creatinine, gamma-aminobutyric-lysine, oleic acid, glycine, poly-γ-glutamic acid, and eicosapentaenoic acid in VAWEs might be involved in ameliorating the symptoms of colitis. This study provided evidence for the potential usage of SVAE and RVAE as anti-colitis agents. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175111390&doi=10.3390%2fbiomedicines11071913&partnerID=40&md5=cae1449d95a75d27dbe7cc607fecd572 https://scholars.lib.ntu.edu.tw/handle/123456789/637264 |
ISSN: | 2227-9059 | DOI: | 10.3390/biomedicines11071913 |
顯示於: | 動物科學技術學系 |
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