https://scholars.lib.ntu.edu.tw/handle/123456789/637356
標題: | DEVELOPMENT OF MICROWELL ARRAY FOR CELL TRAPPING AND SHRNA DELIVERY USING ELECTROPORATION | 作者: | Hsieh, Han Yun Lee, Che Yi Chao, Jian Chie Fan, Yu Jui HORN-JIUNN SHEEN |
關鍵字: | Dielectrophoresis | Electroporation | ITO glass | MEMS | shRNA delivery | 公開日期: | 1-一月-2022 | 來源出版物: | MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences | 摘要: | First, we use microelectromechanical system (MEMS) technology to fabricate devices with different geometric structures on indium tin oxide (ITO) glass. The devices will be connected to an external electrical signal source to make the cells captured into the etched holes with negative dielectrophoresis (nDEP) to achieve cell manipulation. Second, we modulate the frequency of the electrical signal to achieve cell electroporation, and stain the cells with CFDA-SE and calcein to verify that after electropration, substances can be delivered from inside or outside the cell, and to find the optimal device for shRNA delivery. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/637356 | ISBN: | 9781733419048 |
顯示於: | 應用力學研究所 |
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