https://scholars.lib.ntu.edu.tw/handle/123456789/638155
標題: | Visualizing the DNA repair process by a photolyase at atomic resolution | 作者: | MANUEL MAESTRE-REYNA Wang, Po-Hsun Nango, Eriko Hosokawa, Yuhei Saft, Martin Furrer, Antonia Yang, Cheng-Han Gusti Ngurah Putu, Eka Putra Wu, Wen-Jin Emmerich, Hans-Joachim Caramello, Nicolas Franz-Badur, Sophie Yang, Chao Engilberge, Sylvain Wranik, Maximilian Glover, Hannah Louise Weinert, Tobias Wu, Hsiang-Yi Lee, Cheng-Chung Huang, Wei-Cheng Huang, Kai-Fa Chang, Yao-Kai Liao, Jiahn-Haur Weng, Jui-Hung Gad, Wael Chang, Chiung-Wen Pang, Allan H Yang, Kai-Chun Lin, Wei-Ting Chang, Yu-Chen Gashi, Dardan Beale, Emma Ozerov, Dmitry Nass, Karol Knopp, Gregor Johnson, Philip J M Cirelli, Claudio Milne, Chris Bacellar, Camila Sugahara, Michihiro Owada, Shigeki Joti, Yasumasa Yamashita, Ayumi Tanaka, Rie Tanaka, Tomoyuki Luo, Fangjia Tono, Kensuke Zarzycka, Wiktoria Müller, Pavel Alahmad, Maisa Alkheder Bezold, Filipp Fuchs, Valerie Gnau, Petra Kiontke, Stephan Korf, Lukas Reithofer, Viktoria Rosner, Christian Joshua Seiler, Elisa Marie Watad, Mohamed Werel, Laura Spadaccini, Roberta Yamamoto, Junpei Iwata, So Zhong, Dongping Standfuss, Jörg Royant, Antoine Bessho, Yoshitaka Essen, Lars-Oliver Tsai, Ming-Daw |
公開日期: | 十二月-2023 | 卷: | 382 | 期: | 6674 | 來源出版物: | Science (New York, N.Y.) | 摘要: | Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638155 | ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.add7795 |
顯示於: | 化學系 |
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