https://scholars.lib.ntu.edu.tw/handle/123456789/638234
標題: | Co-printing of micro/nanostructures integrated with preconcentration to enhance protein detection | 作者: | Lu, Yi Jung Hsieh, Han Yun Yang, Wen Fai KUANG-CHONG WU Tahara, Hidetoshi Wei, Pei Kuen HORN-JIUNN SHEEN Fan, Yu Jui |
關鍵字: | Biosensor | Hot embossing | Ion concentration polarization | Localized surface plasmon resonance (LSPR) | Microfluidics | 公開日期: | 1-一月-2024 | 卷: | 28 | 期: | 1 | 來源出版物: | Microfluidics and Nanofluidics | 摘要: | This paper reports a fabrication method that can make microstructures such as microfluidic channels and nanostructures to generate surface plasmon resonance (SPR) signals in one-step using hot embossing. We first made a micro/nanostructural mold on a silicon substrate through sequential e-beam lithography, reactive ion etching (RIE), photolithography, and inductively coupled plasma RIE. The fabricated mold and cyclo-olefin polymer (COP) film were pressed between two flat, heated metal bases under optimal conditions, and the micro/nanostructures were complementarily transferred to the COP film. After depositing a thin aluminum film onto the nanostructure, the device was completed by patterning Nafion that crossed two channels and a nearby nanostructure, and by bonding the COP film to a flat polydimethylsiloxane (PDMS) substrate with holes punched for the inlets and outlets. SPR signals of the nanostructures of the microfluidic channel were calibrated using glycerol solutions of different percentages, and a wavelength sensitivity of 393 nm/refractive index unit was found for the Al-based nanoslit SPR sensing chip. To detect macromolecules, we first modified bovine serum albumin (BSA) onto the surface of the SPR chip and then allowed different concentrations of anti-BSA samples to flow into the device. A calibration curve for detecting anti-BSA was constructed, and anti-BSA detection levels with and without preconcentration were compared. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638234 | ISSN: | 16134982 | DOI: | 10.1007/s10404-023-02699-4 |
顯示於: | 應用力學研究所 |
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