https://scholars.lib.ntu.edu.tw/handle/123456789/638576
標題: | Second-harmonic generation investigation of collagen thermal denaturation | 作者: | Chen, Wei Liang Sun, Yen SUNG-JAN LIN SHIOU-HWA JEE YANG-FANG CHEN Lin, G. Chih So, Peter T.C. CHEN-YUAN DONG |
關鍵字: | Collagen | Second harmonic generation (SHG) | Thermal denaturation | 公開日期: | 31-八月-2007 | 卷: | 6442 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | Using the technique of second-harmonic generation (SHG) microscopy we obtained large area image of type I collagen from rat tail tendon as it is heated from 40°C to 70°C for 0 to 180 minutes. The high resolution images allowed us to investigate the collagen structural change. We observed that heating the tendon below the temperature of 54°C does not produce any change in the averaged SHG intensity. At the heating temperature of 54°C and above, we find that increasing the heating temperature and time leads to decreasing SHG intensity. As the tendon is heated above 54°C, a decrease in the SHG signal occurs uniformly throughout the tendon, but the regions where the SHG signal vanishes form a tiger-tail like pattern. By comparing the relative SHG intensities in small and large areas, we found that the denaturation process responsible for forming the tiger-tail like pattern occurs at a higher rate than the global denaturation process occurring throughout the tendon. Our results show that second-harmonic generation microscopy is effective in monitoring the thermal damage to collagen and has potential applications in biomedicine. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638576 | ISBN: | 0819465550 | ISSN: | 16057422 | DOI: | 10.1117/12.698524 |
顯示於: | 醫學工程學研究所 |
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