https://scholars.lib.ntu.edu.tw/handle/123456789/638767
標題: | Protective effects of systemic treatment with methylprednisolone in a rodent model of non-arteritic anterior ischemic optic neuropathy (rAION) | 作者: | Huang, Tzu-Lun Huang, Shun-Ping Chang, Chung-Hsing Lin, Kung-Hung SHU-WEN CHANG Tsai, Rong-Kung |
關鍵字: | Methylprednisolone; Optic nerve; Retinal ganglion cell; Rodent model of non-arteritic anterior ischemic optic neuropathy; Visual evoked potential | 公開日期: | 二月-2015 | 卷: | 131 | 來源出版物: | Experimental eye research | 摘要: | This study investigated the protective effects of the administration of steroids on optic nerves (ON) and retinal ganglion cells (RGCs) in a rodent model of non-arteritic anterior ischemic optic neuropathy (rAION). We induced rAION using rose bengal and argon laser irradiation in a photodynamic procedure on the optic discs of rats. The treated groups received methylprednisolone (MP) via peritoneal injection for 2 weeks. The control group received intraperitoneal injections of phosphate-buffered saline (PBS) post-rAION. At the 4th week post-infarct, MP treatments significantly rescued the RGCs (mm(2)) in the central retinas (1920 ± 210, p < 0.001) and mid-peripheral retinas (950 ± 240, respectively, p = 0.018) compared with those of the PBS-treated rats (central: 900 ± 210 and mid-peripheral: 440 ± 180). Functional assessment with flash visual-evoked potentials demonstrated that P1 latency (ms) was shortened in the MP group compared to the PBS group (108 ± 14 and 147 ± 9, respectively, p < 0.001). In addition, the P1 amplitude (uV) was enhanced in the MP group compared to the PBS group (55 ± 12 and 41 ± 13, respectively, p < 0.05). TUNEL assays showed a decrease in the number of apoptotic cells in the RGC layers of MP-treated retinas compared to the PBS-treated group (p < 0.05). ED1 positive cells (/HPF) were significantly decreased in the ONs of the MP group compared to the PBS group (p < 0.001). In conclusion, systemic administration of MP had neuroprotective effects on RGC survival and ON function in the rAION animal model. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638767 | ISSN: | 00144835 | DOI: | 10.1016/j.exer.2014.12.014 |
顯示於: | 醫學系 |
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