https://scholars.lib.ntu.edu.tw/handle/123456789/638880
標題: | In vitro Biosystems-Stem Cell-based Organ on Chips | 作者: | Lee, I. Chi NAI-CHEN CHENG Shen, Chia Ning MIN-HUEY CHEN Huang, Wen Yen SUNG-JAN LIN Li, Yi Chen Ethan |
公開日期: | 1-一月-2023 | 來源出版物: | Stem Cell-based Biosystems | 摘要: | This chapter discusses the value of the new approach to scientists in basic and applied research. It introduces the innovations and development of different types of tissue/organ-like construction techniques such as in vitro spheroid and organoid. The stem cell-based organoid system develops the right culture conditions to induce stem cells to self-renew into organ- or tissue-like structures. The development of 3D cell/tissue culture models and microfabrication technique has enabled the creation of a new paradigm, organ on chips. In essence, the generation of induced pluripotent stem cells (iPSC)-derived organoids attempts to replicate the developmental processes and organ regeneration in vitro. Apart from utilizing extracellular matrices (ECM) -dependent methods, it is possible to generate iPSCderived organoids in suspension culture via the formation of embryoid bodies (EB). In addition to ECM- and EB- dependent methods, other methods of generating iPSC derived organoids are being attempted to recapture the multicellular composition of organs; these include the use of air-liquid interface or organ-on-a-chip strategies. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638880 | ISBN: | 9781003822684 | DOI: | 10.1201/9781003130048-7 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。