https://scholars.lib.ntu.edu.tw/handle/123456789/638886
標題: | Involvement of a novel C-terminal kinase domain of Kir6.2 in the K-ATP channel rundown reactivation | 作者: | KUO-LONG LOU Chou, Hsiu Chuan Tsai, Yau Wei Shiau, Yu Shuan Huang, Po Tsang Chen, Ting Yu Shiau, Yuh Yuan French, Robert J. |
關鍵字: | 3D homology modeling | Channel gating | Kinase domain | Kir6.2 | Rundown reactivation | 公開日期: | 1-十二月-2001 | 卷: | 7 | 期: | 4 | 來源出版物: | Journal of Molecular Modeling | 摘要: | Rundown is a generally encountered problem while recording KATP channel activity with inside-out patches. No assigned structural fragment related to this mechanism has yet been derived from any of the functional analyses performed. Therefore, based on a combined sequence and secondary structure alignment against known crystal structure of segments from closely related proteins, we propose here the three-dimensional structural model of an intracellular C-terminal domain of the Kir6.2 subunit in KATP channels. An E. coli CMP-kinase was suggested as template for the model building. The subdomain arrangement of this novel kinase domain and the structural correlation for UDP-docking are described. With structural-functional interpretation, we conclude that the reactivation of KATP channel rundown by MgATP or UDP is very possibly regulated by this intracellular kinase domain at the C-terminus of Kir6.2 subunit in KATP channels. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638886 | ISSN: | 16102940 | DOI: | 10.1007/S008940100010 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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