https://scholars.lib.ntu.edu.tw/handle/123456789/639358
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | KUEN-YUH WU | en_US |
dc.contributor.author | Wei, Yu-Tzu | en_US |
dc.contributor.author | YU-SYUAN LUO | en_US |
dc.contributor.author | Shen, Li-Chin | en_US |
dc.contributor.author | Chang, Bao-Suei | en_US |
dc.contributor.author | Chen, Ya-Yin | en_US |
dc.contributor.author | Huang, Yan-Chi | en_US |
dc.contributor.author | Huang, Hui-Fen | en_US |
dc.contributor.author | Chung, Wen-Sheng | en_US |
dc.contributor.author | Chiang, Su-Yin | en_US |
dc.date.accessioned | 2024-01-30T06:31:46Z | - |
dc.date.available | 2024-01-30T06:31:46Z | - |
dc.date.issued | 2023-11 | - |
dc.identifier.issn | 02786915 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/639358 | - |
dc.description.abstract | Safrole oxide (SAFO), a metabolite of naturally occurring hepatocarcinogen safrole, is implicated in causing DNA adduct formation. Our previous study first detected the most abundant SAFO-induced DNA adduct, N7-(3-benzo[1,3] dioxol-5-yl-2-hydroxypropyl)guanine (N7γ-SAFO-G), in mouse urine using a well-developed isotope-dilution high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (ID-HPLC-ESI-MS/MS) method. This study further elucidated the genotoxic mode of action of SAFO in mice treated with SAFO 30, 60, 90, or 120 mg/kg for 28 days. The ID-HPLC-ESI-MS/MS method detected N7γ-SAFO-G with excellent sensitivity and specificity in mouse liver and urine of SAFO-treated mice. Our data provide the first direct evidence of SAFO-DNA adduct formation in rodent tissues. N7γ-SAFO-G levels in liver were significantly increased by SAFO 120 mg/kg compared with SAFO 30 mg/kg, suggesting rapid spontaneous or enzymatic depurination of N7γ-SAFO-G in tissue DNA. Urinary N7γ-SAFO-G exhibited a sublinear dose response. Moreover, the micronucleated peripheral reticulocyte frequencies increased dose-dependently and significantly correlated with N7γ-SAFO-G levels in liver (r = 0.8647; p < 0.0001) and urine (r = 0.846; p < 0.0001). Our study suggests that safrole-mediated genotoxicity may be caused partly by its metabolic activation to SAFO and that urinary N7γ-SAFO-G may serve as a chemically-specific cancer risk biomarker for safrole exposure. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science Ltd | en_US |
dc.relation.ispartof | Food And Chemical Toxicology | en_US |
dc.subject | Isotope dilution; Liquid chromatography-tandem mass spectrometry; Micronuclei; N7-guanine adduct; Safrole; Safrole oxide | en_US |
dc.title | Dose-response formation of N7-(3-benzo[1,3]dioxol-5-yl-2-hydroxypropyl)guanine in liver and urine correlates with micronucleated reticulocyte frequencies in mice administered safrole oxide | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.fct.2023.114056 | - |
dc.identifier.pmid | 37739051 | - |
dc.identifier.scopus | 2-s2.0-85173441353 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85173441353 | - |
dc.relation.journalvolume | 181 | en_US |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Institute of Food Safety and Health | - |
crisitem.author.dept | Institute of Environmental and Occupational Health Sciences | - |
crisitem.author.dept | Public Health | - |
crisitem.author.dept | Institute of Food Safety and Health | - |
crisitem.author.dept | Master of Public Health Degree Program | - |
crisitem.author.orcid | 0000-0003-1388-475X | - |
crisitem.author.orcid | 0000-0001-5481-6900 | - |
crisitem.author.parentorg | College of Public Health | - |
crisitem.author.parentorg | College of Public Health | - |
crisitem.author.parentorg | College of Public Health | - |
crisitem.author.parentorg | College of Public Health | - |
crisitem.author.parentorg | College of Public Health | - |
顯示於: | 食品安全與健康研究所 |
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