https://scholars.lib.ntu.edu.tw/handle/123456789/639756
標題: | Fipronil disturbs the antigen-specific immune responses and GABAergic gene expression in the ovalbumin-immunized BALB/c mice | 作者: | Kuo, Jui Fang Cheng, Yin Hua Tung, Chun Wei CHIA-CHI WANG |
關鍵字: | Antigen-specific immune responses | Fipronil | GABAergic signaling | Immunotoxicity | Ovalbumin | 公開日期: | 1-十二月-2024 | 卷: | 20 | 期: | 1 | 來源出版物: | BMC Veterinary Research | 摘要: | Background: Fipronil (FPN) is a broad-spectrum pesticide and commonly known as low toxicity to vertebrates. However, increasing evidence suggests that exposure to FPN might induce unexpected adverse effects in the liver, reproductive, and nervous systems. Until now, the influence of FPN on immune responses, especially T-cell responses has not been well examined. Our study is designed to investigate the immunotoxicity of FPN in ovalbumin (OVA)-sensitized mice. The mice were administered with FPN by oral gavage and immunized with OVA. Primary splenocytes were prepared to examine the viability and functionality of antigen-specific T cells ex vivo. The expression of T cell cytokines, upstream transcription factors, and GABAergic signaling genes was detected by qPCR. Results: Intragastric administration of FPN (1–10 mg/kg) for 11 doses did not show any significant clinical symptoms. The viability of antigen-stimulated splenocytes, the production of IL-2, IL-4, and IFN-γ by OVA-specific T cells, and the serum levels of OVA-specific IgG1 and IgG2a were significantly increased in FPN-treated groups. The expression of the GABAergic signaling genes was notably altered by FPN. The GAD67 gene was significantly decreased, while the GABAR β2 and GABAR δ were increased. Conclusion: FPN disturbed antigen-specific immune responses by affecting GABAergic genes in vivo. We propose that the immunotoxic effects of FPN may enhance antigen-specific immunity by dysregulation of the negative regulation of GABAergic signaling on T cell immunity. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85182812689&doi=10.1186%2fs12917-024-03878-3&partnerID=40&md5=f371afbb812c3fdefadb02432b069a8a https://scholars.lib.ntu.edu.tw/handle/123456789/639756 |
ISSN: | 1746-6148 | DOI: | 10.1186/s12917-024-03878-3 |
顯示於: | 獸醫學系 |
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