https://scholars.lib.ntu.edu.tw/handle/123456789/640214
標題: | An alleviative effect of Lonicerae japonicae flos water extract against liver fibrogenesis in vitro and in vivo | 作者: | Lin, Yi Ling Wu, Yi Hsieng Samuel Chao, Ming Yuan Yang, Deng Jye Liu, Cheng Wei Tseng, Jung Kai YI-CHEN CHEN |
關鍵字: | anti-inflammation | antiapoptosis | antioxidation | liver fibrogenesis | Lonicera japonicae flos | 公開日期: | 1-一月-2024 | 來源出版物: | Environmental Toxicology | 摘要: | Lonicerae japonicae (L. japonicae) flos is a medical and food homology herb. This study investigated the phenolic acid and flavonoid contents in L. japonicae flos water extract solution (LJWES) and the preventive effects of LJWES against liver fibrogenesis via FL83B cells and rats. LJWES contains many polyphenols, such as chlorogenic acid, morin, and epicatechin. LJWES increased cell viability and decreased cytotoxicity in thioacetamide (TAA)-treated FL83B cells (75 mM) (p <.05). LJWES decreased (p <.05) gene expressions of Tnf-α, Tnfr1, Bax, and cytochrome c but upregulated Bcl-2 and Bcl-xl in TAA-treated cells; meanwhile, increased protein levels of P53, cleaved caspase 3, and cleaved caspase 9 in TAA treated cells were downregulated (p <.05) by LJWES supplementation. In vivo, results indicated that TAA treatment increased serum liver damage indices (alanine aminotransferase [ALT] and alkaline phosphatase [ALP]) and cytokines (interleukin-6 and transforming growth factor-β1) levels and impaired liver antioxidant capacities (increased thiobarbituric acid reactive substance value but decreased catalase/glutathione peroxidase activities) in rats (p <.05) while LJWES supplementation amended (p <.05) them. Liver fibrosis scores, collagen deposition, and alpha-smooth muscle actin deposition in TAA-treated rats were also decreased by LJWES supplementation (p <.05). To sum up, LJWES could be a potential hepatoprotective agent against liver fibrogenesis by enhancing antioxidant ability, downregulating inflammation in livers, and reducing apoptosis in hepatocytes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183904255&doi=10.1002%2ftox.24154&partnerID=40&md5=eebcaf075d730ff114245cb627084ded https://scholars.lib.ntu.edu.tw/handle/123456789/640214 |
ISSN: | 15204081 | DOI: | 10.1002/tox.24154 |
顯示於: | 動物科學技術學系 |
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