https://scholars.lib.ntu.edu.tw/handle/123456789/641619
標題: | Enhancement of Tumorigenicity, Spheroid Niche, and Drug Resistance of Pancreatic Cancer Cells in Three-Dimensional Culture System | 作者: | Hung, Hao-Chien TSUI-LIEN MAO Fan, Ming-Huei Huang, Guan-Zhi Minhalina, Ainani Priza Chen, Chi-Long Liu, Chao-Lien |
關鍵字: | CXCR3/CXCL10 axis; Pancreatic cancer (PaC); cancer-associated fibroblast (CAF); spheroid model; therapeutic discovery.; three dimensional (3D); two dimensional (2D) | 公開日期: | 2024 | 卷: | 15 | 期: | 8 | 來源出版物: | Journal of Cancer | 摘要: | The three-dimensional (3D) cell culture technique has been applied comprehensively as a variable platform for medical research, biochemical signal pathway analysis, and evaluation of anti-tumor treatment response due to an excellent recapitulation of a tumor microenvironment (TME) in the in vitro cultured cancer cells. Pancreatic cancer (PaC) is one of the toughest malignancies with a complex TME and refractory treatment response. To comprehensively study the TME of PaC, there is an eager need to develop a 3D culture model to decompose the cellular components and their cross interactions. Herein, we establish a 3D PaC culture system with cancer stem cell (CSC) and scalability properties. To validate our model, we tested the individual PaC cell and the combined effects with cancer-associated fibroblasts (CAFs) on cancer tumorigenicity, the cellular interaction through the CXCR3/CXCL10 axis, and cellular responses reflection of anti-cancer treatments. With the help of our 3D technology, a simulated malignant spheroid with important stromal populations and TME physiochemical properties may be successfully recreated. It can be used in a wide range of preclinical research and helpful in advancing basic and translational cancer biology. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641619 | ISSN: | 1837-9664 | DOI: | 10.7150/jca.87494 |
顯示於: | 病理學科所 |
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