https://scholars.lib.ntu.edu.tw/handle/123456789/641652
標題: | Interactions of neural-like cells with 3D-printed polycaprolactone with different inner diameters for neural regeneration | 作者: | Javkhlan, Zolzaya Hsu, Sheng Hao Chen, Rung Shu MIN-HUEY CHEN |
關鍵字: | 3D printing | Half tubular polycaprolactone | Neural regeneration | PC12 | Poly-D-Lysine | 公開日期: | 1-一月-2024 | 來源出版物: | Journal of Dental Sciences | 摘要: | Background/purpose: Peripheral neural regeneration is an interesting and challenging field. The aim of this study was to investigate the interactions of neural-like PC12 cells and Poly-D-Lysine (PDL)-coated 3D-printed polycaprolactone (PCL) scaffolds with different inner diameters of half tubular array (HTA) (0, 200, 300, and 400 μm), respectively. Materials and methods: This study used the fused deposition modeling (FDM) technique with 3D-printing to fabricate the thermoplastic polymer. Scaffold properties were measured by mechanical testing, and coating quality was observed under a scanning electron microscope (SEM). PC12 cell biocompatibility was examined by an MTT assay. Cell differentiation was evaluated by immunofluorescence staining. Results: The cell viability of PC12 cells on PDL-coated PCL scaffolds with a 200-μm inner diameter of HTA was shown with significant differences (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001) than other PCL groups at all experimental dates. The SEM observation showed that PDL-coated PCL scaffolds with 200-μm inner diameters of HTA promoted cell adhesion. An immunofluorescence staining of PC12 cells on the PDL-coated PCL scaffold with a 200-μm inner diameter of the HTA group showed that it stimulated PC12 cells for neurite formation much better than the other groups. Conclusion: A PDL-coated PCL scaffold with a 200-μm inner diameter of HTA can promote the growth and differentiation of PC12 cells better than other groups. It indicated that PDL-coated PCL scaffolds with a 200-μm inner diameter HTA can be used for further neural regeneration application. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641652 | ISSN: | 19917902 | DOI: | 10.1016/j.jds.2023.12.017 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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