https://scholars.lib.ntu.edu.tw/handle/123456789/641794
標題: | Epitope(s) involving amino acids of the fusion loop of Japanese encephalitis virus envelope protein is(are) important to elicit protective immunity | 作者: | YI-CHIN FAN Chen, Jo-Mei Chen, Yi-Ying Ke, Yuan-Dun Chang, Gwong-Jen J Chiou, Shyan-Song |
關鍵字: | Japanese encephalitis virus; fusion loop | 公開日期: | 26-三月-2024 | 出版社: | American Society for Microbiology | 來源出版物: | Journal of Virology | 摘要: | Dengue vaccine candidates have been shown to improve vaccine safety and efficacy by altering the residues or accessibility of the fusion loop on the virus envelope protein domain II (DIIFL) in an ex vivo animal study. The current study aimed to comprehensively investigate the impact of DIIFL mutations on the antigenicity, immunogenicity, and protective efficacy of Japanese encephalitis virus (JEV) virus-like particles (VLPs) in mice. We found the DIIFL G106K/L107D (KD) and W101G/G106K/L107D (GKD) mutations altered the binding activity of JEV VLP to cross-reactive monoclonal antibodies but had no effect on their ability to elicit total IgG antibodies in mice. However, JEV VLPs with KD or GKD mutations induced significantly less neutralizing antibodies against JEV. Only 46% and 31% of the KD and GKD VLPs-immunized mice survived compared to 100% of the wild-type (WT) VLP-immunized mice after a lethal JEV challenge. In passive protection experiments, naïve mice that received sera from WT VLP-immunized mice exhibited a significantly higher survival rate of 46.7% compared to those receiving sera from KD VLP- and GKD VLP-immunized mice (6.7% and 0%, respectively). This study demonstrated that JEV DIIFL is crucial for eliciting potently neutralizing antibodies and protective immunity against JEV. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641794 | ISSN: | 0022-538X 1098-5514 |
DOI: | 10.1128/jvi.01773-23 |
顯示於: | 流行病學與預防醫學研究所 |
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