https://scholars.lib.ntu.edu.tw/handle/123456789/641941
標題: | Development of label-free triboelectric nanosensors as screening platforms for anti-tumor drugs | 作者: | Cheng, Yu Ying Ganguly, Anindita Cheng, Yi Yun Ortiz, Christopher Llynard D. Pal, Arnab Shah, Pramod Kaswan, Kuldeep Yang, Lee Wei ZONG-HONG LIN |
關鍵字: | Chemotherapy | Drug screening | Self-powered sensing | Solid-liquid contact separation | Triboelectric nanosensor | 公開日期: | 15-六月-2024 | 卷: | 125 | 來源出版物: | Nano Energy | 摘要: | This study introduces a general label-free drug screening platform with potential to support high-throughput drug screening for any protein target. Its innovation lies in quantifying the affinity of molecule-molecule interactions via voltage output variation, which achieves unprecedented selectivity and sensitivity. It combines computational analysis complemented by experimental substantiation with a solid-liquid triboelectric nanosensor. Owing to its high binding affinity, FKBP-rapamycin is used as a model system to demonstrate the platform's sensitivity. Subsequently, the research is extended to study drug interactions with an oncogenic protein ATG4B. The findings unveil the binding of S130 and Tioconazole to ATG4B, a critical cysteine protease involved in autophagy, while revealing that Dexamethasone does not bind ATG4B. This binding exerts a specific inhibitory effect on autophagic flux and triggers cancer cell apoptosis. The results support the previously verified inhibitory effects of these drugs and the effectiveness of a newly developed self-powered drug screening platform, leveraging the principles of solid-liquid contact electrification. This approach adeptly confronts enduring challenges in traditional drug development methods where biochemical assays need to be designed for each individual protein target or only time-consuming and concentration-demanding molecular interaction measurements were made available. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641941 | ISSN: | 22112855 | DOI: | 10.1016/j.nanoen.2024.109519 |
顯示於: | 醫學工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。