https://scholars.lib.ntu.edu.tw/handle/123456789/84036
Title: | Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs | Authors: | Tung, Chih-kuan Periasamy, Ammasi So, Peter T. C. Chiu, Tsung-Kai Lo, Wen Wang, Po-Hsiang SUNG-JAN LIN Jee, Shiou-Hwa Dong, Chen-Yuan |
Keywords: | Fluorescence; Imaging; Microscopy; Skin; Spherical aberration; Two-photon | Issue Date: | 2003 | Journal Volume: | 4963 | Start page/Pages: | 95-104 | Source: | Proceedings of SPIE | Abstract: | Index mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/132756 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141605430&doi=10.1117%2f12.478026&partnerID=40&md5=1b0b60371a1342689ac43b2da4bccf1a |
ISSN: | 0277786X | DOI: | 10.1117/12.478026 | SDG/Keyword: | Aberrations; Biomedical engineering; Fluorescence; Microscopic examination; Multiphoton processes; Refractive index; Tissue; Two-photon fluorescence microscopy (TPFM); Medical imaging |
Appears in Collections: | 醫學工程學研究所 |
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