https://scholars.lib.ntu.edu.tw/handle/123456789/84106
標題: | The Effects of Depilatory Agents as Penetration Enhancers on Human Stratum Corneum Structures | 作者: | Lee, Jin-Ning SHIOU-HWA JEE CHIH-CHIEH CHAN Lo, Wen CHEN-YUAN DONG SUNG-JAN LIN |
公開日期: | 2008 | 卷: | 128 | 期: | 9 | 起(迄)頁: | 2240-2247 | 來源出版物: | Journal of Investigative Dermatology | 摘要: | The depilatory cream thioglycolate has been shown to be an effective enhancer for transdermal drug delivery. However the mechanism remains unknown. In addition, it may also increase the risk of permeation of exogenous toxic agents across skin in depilatory cream users. The aim of this study was to characterize its effect on the transepidermal route and the associated structural alterations. Fresh human skin was treated with a depilatory cream for 10 minutes and then permeated with fluorescent model drugs. The penetration of model drugs was then imaged and quantified. The structural alternations of stratum corneum were assessed by multi-photon imaging, histology, Nile red staining, and electron microscopy. Our results show that penetration of both hydrophilic and hydrophobic model drugs across stratum corneum was enhanced. Disruption of cellular integrity and focal detachment of superficial corneocytes was observed in multi-photon imaging. In addition, nile red staining showed disorganized lipid distribution. Finally, ultrastructural analysis revealed disruption of intracellular keratin matrix, protein cell envelope, and regular lamellar intercellular lipid packing. Because intracellular and intercellular structures were altered, our results suggest that depilatory agents enhance transepidermal drug delivery by reducing resistance in both transcellular and intercellular routes of stratum corneum. © 2008 The Society for Investigative Dermatology. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/132839 https://www.scopus.com/inward/record.uri?eid=2-s2.0-49549095807&doi=10.1038%2fjid.2008.82&partnerID=40&md5=999f1b1ea84acaf1c49651fe63f90ab6 |
ISSN: | 0022202X | DOI: | 10.1038/jid.2008.82 | SDG/關鍵字: | thioglycolate calcium; thioglycolic acid derivative; adult; article; cell adhesion; cell structure; controlled study; drug delivery system; electron microscopy; fluorescence angiography; histology; human; human tissue; hydrophilicity; hydrophobicity; immunohistochemistry; multiphoton microscopy; priority journal; skin penetration; stratum corneum |
顯示於: | 醫學工程學研究所 |
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