https://scholars.lib.ntu.edu.tw/handle/123456789/86199
Title: | 行政院國家科學委員會專題研究計畫成果報告:台灣烏腳病地區養殖魚類砷累積之生態毒物模擬(3/3) | Authors: | 廖中明 | Keywords: | 砷;曲線下面積;烏腳病;藥理動力及動態模式;吳郭魚;Arsenic;Area-under-curve;Blackfoot disease;Physiologically based pharmacokinetics;Pharmacodynamics;Tilapia | Issue Date: | 2005 | Publisher: | 臺北市:國立臺灣大學生物環境系統工程學系暨研究所 | Abstract: | 此三年研究計畫主要進行台灣烏腳病地區養殖魚 類砷累積之野外調查及生態毒物模擬,經由發展以 生物為基礎的藥理動力及動態模式探討吳郭魚體 內器官之砷濃度。模式模擬主要標的器官包括魚 肉、鰓、腸壁、消化道及肝臟,各器官間由血液傳 輸。經由標的器官濃度與動態反應關係顯示砷的吸 收、代謝及分布情形,並結合濃度和時間曲線下面 積(AUC)為基礎之急性毒暴露模式描述毒理動 態。模式驗證過程採用養殖池野外調查資料及吸收 排除實驗資料進行比較,結果顯示預測資料與實測 濃度吻合,且模式應用可合理地模擬與建構標的器 官濃度與致死效應之劑量反應關係。經由模擬結果 顯示在短期暴露於砷的過程之中,鰓可以做為一個 具有高敏感度的生物指標。此外,經由整合砷的藥 理動力及動態模式與濃度曲線下面積(AUC)模式 可定量地估測吳郭魚標的器官濃度與動態反應。本 研究建立以生物為基礎之風險評估架構可提供未 來水域生物在不同暴露情境下的重金屬累積模擬。 This proposal focuses on field investigation and ecotoxicological modeling arsenic accumulation in aquacultural fish from blackfoot disease area in Taiwan. A physiologically based pharmacokinetic and pharmacodynamic (PBPK/PD) model was developed for arsenic (As) in tilapia Oreochromis mossambicus. The PBPK/PD model structure consisted of muscle, gill, gut wall, alimentary canal, and liver, which were interconnected by blood circulation. We integrate the target organ concentrations and dynamic response describing uptake, metabolism, and disposition of As and the associated area-under-curve (AUC)-based toxicological dynamics following an acute exposure. The model validations were compared against the field observations from real tilapia farms and previously published uptake/depuration experimental data, indicating predicted and measured As concentrations in major organs of tilapia were in good agreement. The model was utilized to reasonably simulate and construct a dose-dependent dynamic response between mortality effect and equilibrium target organ concentrations. Model simulations suggest that tilapia gills may serve as a surrogate sensitive biomarker of short-term exposure to As. This integrated As PBPK/PD/AUC model quantitatively estimates target organ concentration and dynamic response in tilapia and is a strong framework for future waterborne metal model development and for refining a biologically based risk assessment for exposure of aquatic species to waterborne metals under a variety of scenarios. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/10787 | Other Identifiers: | 932313B002005 | Rights: | 國立臺灣大學生物環境系統工程學系暨研究所 |
Appears in Collections: | 生物環境系統工程學系 |
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932313B002005.pdf | 291.73 kB | Adobe PDF | View/Open |
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