|Title:||菜鴨卵巢濾泡細胞生長及類固醇生成調控機制之研究(1/2)||Authors:||吳兩新||Keywords:||菜鴨;卵巢濾泡;類固醇內泌素;Tsaiya duck;ovarian follicle;steroid hormone||Issue Date:||2003||Publisher:||臺北市：國立臺灣大學動物科學技術學系暨研究所||Abstract:||
本研究第一年擬利用菜鴨（Tsaiya duck;Anas platyrhynchos var. domestica ）卵巢濾泡細胞，建立卵巢濾泡三細胞分離技術，並利用體外培養卵巢濾泡三細胞，以瞭解其孕酮、睪固酮與雌二醇等類固醇，以及類固醇合成酵素3b-HSD、17b-HSD 與P450aromatase 活性，以瞭解菜鴨卵巢濾泡細胞類固醇內泌素之分泌模式。
結果顯示，菜鴨卵巢濾泡中之粒性細胞以合成孕酮為主，睪固酮次之，其基礎分泌量及3b-HSD 與17b-HSD 酵素活性，以即將排卵的F1 最高，漸次下降至成熟度最低的F4/5。雌二醇分泌量極低，且濾泡大小間無顯著差異。內膜細胞主要合成睪固酮及雌二醇，其基礎分泌量及17b-HSD 與P450aromatase 酵素活性，皆以最小濾泡F4/5 為最高，並隨著濾泡的逐漸成熟而下降。內膜細胞並無孕酮分泌能力。濾泡中之外膜細胞以合成雌二醇為主，其基礎分泌量及P450aromatase 酵素活性以F2 為最高;而外膜細胞幾乎無孕酮及睪固酮分泌能力。
The purpose of this study in first year was to establish the isolation technique of
ovarian follicular granulosa, theca interna and theca externa cells in Tsaiya duck
(Tsaiya duck； Anas platyrhynchos var. domestica. Furthermore, in order to establish
steroid hormone secretion model of ovarian follicular cells in Tsaiya duck,
progesterone, testosterone and estradiol secretion as well as steroid-producing enzyme
activities of 3b-HSD, 17b-HSD and P450arom in follicular cells were studied by in
vitro cell culture system.
Results showed that progesterone was the primary steroid produced by
granulosa cell, and testosterone was the secondary one. The progesterone level and
enzyme activities of 3b-HSD and 17b-HSD of granulosa cell increased gradually
with the follicle maturity. The estradiol secretion from granulosa cell was low, and
was not significantly different between follicles. Steroid production capabilities of
theca interna cell presented in testosterone and estradiol. The F4/5 follicles exhibited
the highest basal testosterone and estradiol secretion as well as enzyme activities of
17b-HSD and P450arom from theca interna cell. Both of them were declined with the
follicle maturity. Besides, theca interna did not secrete progesterone. Estradiol was
the main steroid secreted by theca externa. Theca externa of F2 presented the highest
estradiol secretion and P450arom activity. Moreover, theca externa did not secrete
progesterone and testosterone.
This study demonstrated the different abilities of steroid secretion among
ovarian follicular granulosa, theca interna and theca externa cells in Tsaiya duck by
individual in vitro culture system. However, the relationship between cellular
interaction and steroidogenesis pathway is required further study.
|Appears in Collections:||動物科學技術學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.