https://scholars.lib.ntu.edu.tw/handle/123456789/87849
Title: | 飼糧二十二碳六烯酸對豬隻脂質代謝基因表現之影響(1/3) | Authors: | 丁詩同 | Keywords: | ADD1;DHA;SREBP1 | Issue Date: | 2003 | Publisher: | 臺北市:國立臺灣大學動物科學技術學系暨研究所 | Abstract: | 飼糧中脂肪酸可被動物利用而貯存於各種組織,餵飼含不同脂肪酸的脂質 來源可顯著改變豬隻脂肪組織、肌肉組織和肝臟中脂肪酸的組成(Innis et al., 1996;Ding et al., 2003)。許(2002)於飼糧中添加2% DHA 油,顯著增加豬隻 各組織中DHA 之含量,並顯著抑制仔豬肝臟中與脂質新生合成相關的轉錄因子 Sterol Regulatory Element-Binding Protein-1(SREBP-1) mRNA 的表現。 本研究的目的在餵飼豬隻高DHA、高n-6 多不飽和脂肪酸或高硬脂酸飼 糧,測試不同脂肪酸組成對豬隻血液性狀與基因表現的影響。結果發現餵飼10 %富含n-3 多不飽和脂肪酸之藻類DHA 油飼糧兩天,即能顯著減少仔豬血漿中, 總膽固醇與三酸甘油酯的含量(P<0.001),降低效果分別可達39%與46%,另 一n-6 多不飽和脂肪酸處理組(大豆油組;富含亞麻油酸),則亦顯著降低血漿 中三酸甘油酯的含量(P<0.05),而不影響膽固醇的含量。北方吸漬分析中發現, 添加海藻DHA油可抑制SREBP-1在肝臟中的表現,並顯著促進肝臟ACO mRNA 之表現量,而大豆油的添加並不影響此二基因的表現。由於SREBP-1 可調節許 多參與脂肪生合成基因的表現,餵飼n-3 族多不飽和脂肪酸(DHA)似可因降低 SREBP-1,而抑制體內脂質生合成路徑。另外,增加與脂肪酸氧化基因ACO 的 表現,應可增加體內對脂肪酸的氧化,進而減少豬隻體脂的堆積。所以餵飼DHA 油應可提供一個改善營養分利用,並生產高DHA 肉品之策略。 Dietary fatty acid composition can change fatty acid profiles in several porcine tissues, such as adipose tissue, muscle, and liver(Innis et al., 1996;Ding et al., 2003). Hsu (2002) demonstrated 2% DHA oil in the diet will significantly increased the DHA in several porcine tissues. Such changes also inhibited the expression of hepatic Sterol Regulatory Element-Binding Protein-1(SREBP-1) mRNA . The purpose of current project was to test the effect of dietary high DHA, high n-6 polyunsaturated fatty acid (PUFA), and n-3 PUFA on blood composition and gene expression in several tissues. Dietary DHA supplementation for two d decreased plasma triacylglycerol (TG) and cholesterol concentration(P<0.001). Dietary soybean oil treatment decreased plasma TG but not cholesterol concentration. Northern analysis demonstrated that DHA treatment reduced the SREBP1 mRNA concentration in the liver. The DHA treatment also increased the ACO mRNA concentration. Soybean oil treatment did not have the same effect. The SREBP1 regulates the expression of several lipogenic genes. Therefore, dietary DHA treatment may reduce lipogenic pathway due to inhibition of SREBP1 expression. Taken together, dietary DHA treatment may improve nutrients utilization and produce high DHA meat products. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/16460 | Other Identifiers: | 912313B002405 | Rights: | 國立臺灣大學動物科學技術學系暨研究所 |
Appears in Collections: | 動物科學技術學系 |
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912313B002405.pdf | 152.82 kB | Adobe PDF | View/Open |
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