https://scholars.lib.ntu.edu.tw/handle/123456789/87850
Title: | 飼糧二十二碳六烯酸對豬隻脂質代謝基因表現之影響(2/3) | Authors: | 丁詩同 | Keywords: | 二十二碳六烯酸;抑制性雜合扣除法;基因表現;Dietary fatty acid composition can change fatty acid profiles in several porcine tissues. Hsu (2002) demonstrated 2% DHA oil in the diet will significantly increased the DHA in several porcine tissues. Such changes also inhibited the expression of hepatic Sterol Regulatory Element-Binding Protein-1 ( SREBP-1 ) mRNA . The purpose of current project was to test the effect of dietary high DHA and n-3 PUFA on FA composition and gene expression in the liver. Dietary DHA supplementation for 18 d increased the expression of gene related to FA oxidation. The suppression subtractive hybridization technique was used to detect hepatic genes differentially expressed in the DHA or tallow treated pigs. A differential screening procedure confirmed that there were 12 known genes and 3 novel genes differentially expressed. They were alcohol dehydrogenase, dehydrogenase/ reductase (SDR family) member 8, serum amyloid protein, serine protease inhibitor I, fibrinogen gamma polypeptide, vitamin D binding protein, α1-microglobulin-bikunin precursor, glutathione S-transferase, alpha-1 acid glycoprotein, X-box binding protein pseudogene 1, H19 gene and hypothetical protein MGC56918. Taken together, dietary DHA treatment may be used to produce high DHA meat products and to regulate gene expression. | Issue Date: | 2004 | Publisher: | 臺北市:國立臺灣大學動物科學技術學系暨研究所 | Abstract: | 飼糧中脂肪酸可被動物利用而貯存於 各種組織。許(2002)於飼糧中添加2% 二 十二碳六烯酸DHA 油,顯著增加豬隻各組織 中DHA 之含量,並顯著抑制仔豬肝臟中與脂 質新生合成相關的轉錄因子Sterol Regulatory Element-Binding Protein-1(SREBP-1) mRNA 的表現。本研究的目的在餵飼豬隻高DHA 或 高硬脂酸飼糧,測試不同脂肪酸組成對豬隻 基因表現的影響。結果發現飼糧中添加2% DHA 油18 天,顯著增加豬隻各肝臟中DHA 之含量,並顯著增進豬隻肝臟中與脂質氧化 有關基因的表現。利用抑制性雜合扣除法發 現飼糧中添加DHA 可刺激一些與脂質代謝 相關之新穎基因的表現,本計畫亦初步利用 差異篩選法確認12 已知基因和3 個新的基因 表現隨著飼糧中不同的DHA 而有所不同。這 些已知基因包括:alcohol dehydrogenase、 dehydrogenase/reductase (SDR family) member 8、serum amyloid protein、serine protease inhibitor I、fibrinogen gamma polypeptide、vitamin D binding protein、 α1-microglobulin-bikunin precursor 、 glutathione S- transferase、alpha-1 acid glycoprotein 、X-box binding protein pseudogene 1、H19 gene 和hypothetical protein MGC56918。所以餵飼DHA 油除可生 產高DHA 肉品,亦可影響許多肝臟中基因的 表現。 |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/16462 | Other Identifiers: | 922313B002127 | Rights: | 國立臺灣大學動物科學技術學系暨研究所 |
Appears in Collections: | 動物科學技術學系 |
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922313B002127.pdf | 174.59 kB | Adobe PDF | View/Open |
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