https://scholars.lib.ntu.edu.tw/handle/123456789/92862
標題: | Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles | 作者: | Huang, Pung-Ling Do, Yi-Yin Chana, H.T. Huang, P.L. Chan, T.S. RU-SHI LIU Huang, C.Y. Yang, S.Y. Horng, H.E. |
關鍵字: | Avidin; Bio-compatible nanoparticles; Biotinylated alkaline phosphatase; Hybridization | 公開日期: | 2006 | 卷: | 304 | 期: | 1 | 起(迄)頁: | e415-e417 | 來源出版物: | Journal of Magnetism and Magnetic Materials | 摘要: | In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin. © 2006 Elsevier B.V. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-33646863030&partnerID=MN8TOARS http://ntur.lib.ntu.edu.tw//handle/246246/181300 http://ntur.lib.ntu.edu.tw/bitstream/246246/181300/1/32.pdf |
DOI: | 10.1016/j.jmmm.2006.01.126 |
顯示於: | 園藝暨景觀學系 |
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