https://scholars.lib.ntu.edu.tw/handle/123456789/98160
標題: | Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low-density lipoprotein | 作者: | Lei, Yen-Ping Liu, Cheng-Tzu Sheen, Lee-Yan Chen, Haw-Wen Lii, Chong-Kuei |
關鍵字: | Diallyl disulfide; Diallyl trisulfide; Endothelial nitric oxide synthase; Proteasome; Protein kinase B | 公開日期: | 2010 | 起(迄)頁: | S42-S52 | 來源出版物: | Molecular Nutrition & Food Research | 摘要: | Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox-LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox-LDL, and wortmannin abolished the reversal by DADS and DATS. Similarly, the inhibition of cellular cGMP and nitric oxide production by ox-LDL was reversed by DADS and DATS (p<0.05). This increase in nitric oxide bioavailability by the allyl sulfides was attenuated by wortmannin. Immunoprecipitation assay revealed that DADS and DATS preserved the interaction of eNOS with caveolin-1 in the membrane. In addition, DADS and DATS suppressed the reduction of the cellular eNOS protein content by ox-LDL. When cycloheximide was added to block protein synthesis, DADS and DATS suppressed eNOS protein degradation similarly to that noted by MG132. Ox-LDL increased chymotrypsin-like proteasome activity, and this increase was inhibited by the allyl sulfides and MG132 (p<0.05). These results suggest that DADS and DATS protect eNOS activity against ox-LDL insult. This protection can be attributed partly to their mediation of phosphatidylinositol 3-kinase/protein kinase B signaling and prevention of eNOS degradation. ? 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/190059 | DOI: | 10.1002/mnfr.200900278 | SDG/關鍵字: | allyl compound; caveolin 1; cyclic GMP; diallyl disulfide; diallyl trisulfide; disulfide; endothelial nitric oxide synthase; low density lipoprotein; messenger RNA; oxidized low density lipoprotein; primer DNA; proteasome; sulfide; article; blood; drug effect; garlic; genetics; human; metabolism; physiology; reverse transcription polymerase chain reaction; umbilical vein; vascular endothelium; Allyl Compounds; Caveolin 1; Cyclic GMP; Disulfides; DNA Primers; Endothelium, Vascular; Garlic; Humans; Lipoproteins, LDL; Nitric Oxide Synthase Type III; Proteasome Endopeptidase Complex; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sulfides; Umbilical Veins; Allium sativum |
顯示於: | 食品科技研究所 |
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